As three-dimensional microscopy becomes commonplace in biological research, there is an increasing need for researchers to be able to view experimental image stacks in a natural three-dimensional viewing context. Through stereoscopy and motion tracking, commercial virtual reality headsets provide a solution to this important visualization challenge by allowing researchers to view volumetric objects in an entirely intuitive fashion. With this motivation, we present DIVA, a user-friendly software tool that automatically creates detailed three-dimensional reconstructions of raw experimental image stacks that are integrated in virtual reality. In DIVA's immersive virtual environment, users can view, manipulate and perform volumetric measurements on their microscopy images as they would to real physical objects. In contrast to similar solutions, our software provides high-quality volume rendering with native TIFF file compatibility. We benchmark the software with diverse image types including those generated by confocal, light-sheet and electron microscopy. DIVA is available at https://diva.pasteur.fr and will be regularly updated.

随着三维显微镜在生物学研究中变得司空见惯，研究人员越来越需要能够在自然的三维观察环境中观察实验图像堆栈。通过立体镜和运动跟踪，商用虚拟现实耳机通过允许研究人员以完全直观的方式查看体积对象，从而为这一重要的可视化挑战提供了解决方案。出于这种动机，我们介绍了DIVA，这是一种用户友好的软件工具，可以自动创建集成在虚拟现实中的原始实验图像堆栈的详细三维重建。在DIVA的沉浸式虚拟环境中，用户可以像查看真实物理对象一样在其显微图像上查看，操作和执行体积测量。与类似的解决方案相比，我们的软件可提供具有本机TIFF文件兼容性的高质量体积渲染。我们使用多种图像类型对软件进行基准测试，包括通过共焦，光片和电子显微镜生成的图像类型。DIVA可在https://diva.pasteur.fr上获得，并将定期更新。