Abstract A method for simultaneous analysis of common oxo-arsenic species and their thio-analogues by coupling ion-pair liquid chromatography to inductively coupled plasma mass spectrometry was developed and validated. The chromatographic separation was performed using a C8 stationary phase and a mobile phase containing 1.2 mmol/L tetramethylammonium hydroxide, 4 mmol/L malonic acid, 6.2 mmol/L sodium butane-1-sulfonate, and 0.05 % (v/v) methanol. Under these conditions, the 11 arsenic species (arsenite, arsenate, dimethylarsinic acid, monomethylarsonic acid, arsenobetaine, arsenocholine, tetramethylarsonium cation, trimethylarsine oxide, dimethyldithioarsinic acid, monomethylmonothioarsinic acid, and trimethyarsine sulfide) were separated. The detection limits for these species ranged from 1.4 to 4.0 ng/g As. The method was applied to the analysis of fish, crustacean, mussel, cephalopod and mushroom samples. Thio-arsenic species DMDTAV were found in mussel Mytilus chilensis and cuttlefish Sepis pharaonis samples in very low contents of 23 and 21 ng/g As, respectively. All seafood samples contained predominantly arsenobetaine (330–8570 ng/g As); other As species were present at various contents from 7 to 325 ng/g As.

中文翻译：

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使用 HPLC-ICP-MS 同时测定氧代砷和硫代砷

摘要 建立并验证了一种通过离子对液相色谱与电感耦合等离子体质谱联用同时分析常见含氧砷物种及其硫代类似物的方法。使用 C8 固定相和含有 1.2 mmol/L 四甲基氢氧化铵、4 mmol/L 丙二酸、6.2 mmol/L 丁烷-1-磺酸钠和 0.05% (v/v) 甲醇的流动相进行色谱分离。在这些条件下，11 种砷（亚砷酸盐、砷酸盐、二甲基砷酸、单甲基砷酸、砷甜菜碱、砷胆碱、四甲基砷阳离子、三甲基氧化胂、二甲基二硫代砷酸、单甲基单硫代砷酸和硫化三甲基砷）被分离出来。这些物质的检出限为 1.4 至 4.0 ng/g As。该方法适用于鱼类、甲壳类动物、贻贝、头足类动物和蘑菇样品的分析。在贻贝 Mytilus chilensis 和墨鱼 Sepis pharaonis 样品中发现硫砷物种 DMDTAV，其 As 含量非常低，分别为 23 和 21 ng/g。所有海鲜样品主要含有砷甜菜碱（330–8570 ng/g As）；其他 As 物质的含量从 7 到 325 ng/g As 不等。