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Selection and application of antifungal VOCs-producing yeasts as biocontrol agents of grey mould in fruits.
Food Microbiology ( IF 5.3 ) Pub Date : 2020-06-06 , DOI: 10.1016/j.fm.2020.103556
Santiago Ruiz-Moyano 1 , Alejandro Hernández 1 , Ana I Galvan 2 , María G Córdoba 1 , Rocio Casquete 1 , Manuel J Serradilla 3 , Alberto Martín 1
Affiliation  

Rotting caused by grey mould (Botrytis cinerea) is a concerning disease for numerous crops both pre- and postharvest stages. Application of antagonistic yeasts is a promising strategy for controlling grey mould incidence which could mitigate undesirable consequences of using synthetic fungicides. In this work, a screening for detection of yeasts isolated from figs producers of antifungal volatile organic compounds (VOCs) were performed by confrontation in double dishes systems. Eleven out of 34 yeasts confronted reduced B. cinerea growth parameter in vitro. This reduction was correlated (p ≤ 0.050) with the production of 10 volatile compounds: two acids (acetic acid and octanoic acid), 7 esters (Ethyl propionate, n-Propyl acetate, Isobutyl acetate, 2-methylbutyl acetate, furfuryl acetate, phenylmethyl acetate, 2-phenylethyl acetate) and one ketone (Heptan-2-one). In bases on in vitro assay, Hanseniaspora uvarum 793 was applied to in vivo assays with strawberries and cherries. The reduction of incidence of B. cinerea in strawberries at 7 °C and 25 °C was 54.9 and 72.1% after 6 and 3 days, respectively. The reduction of incidence of B. cinerea in cherries at 7 °C and 25 °C was 48.9 and 45.6% after 5 and 4 days, respectively. These results showed that VOCs produced by Hanseniaspora uvarum 793 are effective in the control of incidence of Botrytis cinerea in fruits, being a potential alternative to chemical fungicide.



中文翻译:

产生抗真菌VOCs的酵母作为水果中灰霉病生物防治剂的选择和应用。

灰霉病灰葡萄孢)引起的腐烂是收获前和收获后许多作物的令人关注的疾病。拮抗酵母菌的应用是控制灰霉病发生率的一种有前途的策略,它可以减轻使用合成杀真菌剂的不良后果。在这项工作中,通过在双皿系统中进行对抗,对从抗真菌挥发性有机化合物(VOC)的无花果生产者分离的酵母进行检测筛选。34种酵母菌中有11种面临灰霉菌的减少体外生长参数。这种减少与(p≤0.050)与10种挥发性化合物的产生相关:两种酸(乙酸和辛酸),7种酯(丙酸乙酯,乙酸正丙酯,乙酸异丁酯,乙酸2-甲基丁酯,乙酸糠酯,苯甲基乙酸酯,乙酸2-苯乙基酯)和一个酮(庚烷-2-酮)。基于体外测定,将Hanseniaspora uvarum 793用于草莓和樱桃的体内测定。6天和3天后,在7°C和25°C的草莓中,B。cinerea的发生率降低分别为54.9和72.1%。灰葡萄孢的发生率降低5天和4天后,在7°C和25°C的樱桃中分别为48.9和45.6%。这些结果表明,由Hanseniaspora uvarum 793产生的挥发性有机化合物可有效控制果实中灰葡萄孢的发生,是化学杀真菌剂的潜在替代品。

更新日期:2020-06-06
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