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Tetraphenylethene-based polymeric fluorescent probes for 2,4,6-trinitrophenol detection and specific lysosome labelling
Dyes and Pigments ( IF 4.1 ) Pub Date : 2020-06-06 , DOI: 10.1016/j.dyepig.2020.108588
Jinya Yao , Zeyan Zhuang , Hongming Yao , Ruijie Shi , Chenyang Chang , Jian Zhou , Zujin Zhao

A series of neutral conjugated polymers (NCPs) and conjugated polyelectrolytes (CPEs) containing tetraphenylethene (TPE) were synthesized and characterized. Due to the presence of TPE units, NCPs exhibit aggregation-induced emission characteristics and strong fluorescence in the aggregated state. NCPs can function as fluorescent probes to detect 2,4,6-trinitrophenol in aqueous media with extremely high sensitivity, low detection limit and good anti-photobleaching properties. The value of quenching constants (Ksv) is by up to 1.72 × 107 M−1 and the detection limit is as low as 5 nM. NCPs can also be made into the fluorescence test strips for the detection of 2,4,6-trinitrophenol in a practical application. The corresponding CPEs are obtained from the facile reaction of the NCPs with pyridines. The CPEs exhibit low cytotoxicity and can be used to track lysosomes in HeLa cells, which is meaningful in visualizing and understanding intracellular metabolism and various physiological processes. Based on the identical polymer skeleton, these conjugated polymers could be employed as fluorescent probes for multipurpose application.



中文翻译:

基于四苯乙烯的聚合荧光探针,用于2,4,6-三硝基苯酚检测和特异性溶酶体标记

合成并表征了一系列中性共轭聚合物(NCP)和含有四苯乙烯(TPE)的共轭聚电解质(CPE)。由于存在TPE单元,NCP在聚集状态下表现出聚集诱导的发射特性和强荧光。NCP可以用作荧光探针,以极高的灵敏度,低的检测限和良好的抗光漂白性能检测水性介质中的2,4,6-三硝基苯酚。淬灭常数(Ksv)的值最大为1.72×10 7  M -1检测限低至5 nM。在实际应用中,也可以将NCP制成荧光测试条,以检测2,4,6-三硝基苯酚。相应的CPE从NCP与吡啶的简便反应中获得。CPE具有较低的细胞毒性,可用于追踪HeLa细胞中的溶酶体,这对于可视化和了解细胞内代谢和各种生理过程具有重要意义。基于相同的聚合物骨架,这些共轭聚合物可用作多用途荧光探针。

更新日期:2020-06-25
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