In this study, the physicochemical characterization of lysozyme adsorbed on gold was investigated. Through the use of MP-SPR it was possible to establish that the orientation of molecules changes from side-on to between or end-on with increasing surface coverage. The data confirms that the process of adsorption is driven primarily by electrostatic interactions but also by hydrophobic forces. MP-SPR data was compared with the Random Sequential Adsorption model for a molecule with an ellipsoidal shape. Contact angle measurements showed that higher surface coverage also translates in more hydrophilic properties of obtained lysozyme layer. Comparison of CD and PM-IRRAS spectra in solution and adsorbed state respectively showed changes in the secondary structures of lysozyme. These changes are dependent on pH, but fundamentally they go in the direction of the increase of β-turn/random content with a simultaneous decrease in β-sheet fraction, which suggests that aggregation is not occurring. The combination of MP-SPR and QCM-D measurements allowed the estimation of the number of water molecules associated with the lysozymes films. It has been observed that hydration decreases from 70% in pH = 4 to 30% in pH = 11. This data indicates that hydration is driven mainly by the degree of protonation of lysozyme molecules.

在这项研究中，研究了吸附在金上的溶菌酶的理化特性。通过使用MP-SPR，可以确定分子的方向从侧面变化到中间或末端变化随着表面覆盖率的增加。数据证实吸附过程主要由静电相互作用驱动，但也受疏水力驱动。将MP-SPR数据与具有椭圆形分子的随机顺序吸附模型进行比较。接触角测量表明，较高的表面覆盖率也转化为获得的溶菌酶层的更亲水的性质。溶液状态和吸附状态下CD和PM-IRRAS光谱的比较分别显示了溶菌酶二级结构的变化。这些变化取决于pH，但从根本上说，它们沿β-转角/无规含量增加的方向，同时β-折叠比例降低，这表明未发生聚集。MP-SPR和QCM-D测量的结合可以估算与溶菌酶薄膜相关的水分子的数量。已经观察到水合从pH ＝ 4的70％降低至pH ＝ 11的30％。该数据表明水合主要由溶菌酶分子的质子化程度驱动。