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Catalytic performance of ceria fibers with phosphatase-like activity and their application as protein carriers
Advanced Powder Technology ( IF 4.2 ) Pub Date : 2020-06-06 , DOI: 10.1016/j.apt.2020.05.016
Katsuya Kato , Sungho Lee , Fukue Nagata

Ceria (CeO2) synthesized by cerium nitrate hexahydrate in alkaline solution under hydrothermal treatment produces a fiber structure that allows high O-P bond cleavage activity. Brunauer–Emmett–Teller (BET) analysis revealed that fiber-morphological CeO2 with high surface area (73.9 m2/g) and pore volume (0.42 cm3/g) showed better hydrolytic activity than nanopolyhedral and cubic morphologies. The CeO2 fiber displayed hydrolytic activity in a tris(hydroxymethyl)aminomethane (Tris) buffer; however, no reaction occurred in a phosphate buffer. From analysis by based on scanning transmission electron microscopy (STEM) and energy-dispersive X-ray spectroscopy (EDX), the phosphate group in the buffer was seen to be immediately adsorbed on the surface of CeO2 particles; therefore, the CeO2 catalysts could not attack the phosphoric esters as a substrate. In addition, the CeO2 fiber showed hydrolytic activity for deoxyribonucleic acid (DNA). Moreover, enzymes loaded on the CeO2 fiber particles retained activity levels equivalent to free-solution enzymes. It is thought that the findings of the present study regarding the properties of CeO2 fiber will have a significant impact in the fields of not only antibacterial and antimicrobial reagents, but also biosensors and biocatalysts.



中文翻译:

具有磷酸酶样活性的二氧化铈纤维的催化性能及其作为蛋白质载体的应用

由六水合硝酸铈在碱性溶液中经水热处理合成的二氧化铈(CeO 2)产生的纤维结构具有很高的OP键裂解活性。Brunauer–Emmett–Teller(BET)分析显示,具有高表面积(73.9 m 2 / g)和孔体积(0.42 cm 3 / g)的纤维形态CeO 2显示出比纳米多面体和立方形态更好的水解活性。首席执行官2纤维在三(羟甲基)氨基甲烷(Tris)缓冲液中表现出水解活性;然而,在磷酸盐缓冲液中没有反应发生。通过基于扫描透射电子显微镜(STEM)和能量色散X射线光谱法(EDX)的分析,发现缓冲液中的磷酸基团立即吸附在CeO 2颗粒的表面上。因此,CeO 2催化剂不能侵蚀磷酸酯作为底物。另外,CeO 2纤维表现出对脱氧核糖核酸(DNA)的水解活性。此外,负载在CeO 2纤维颗粒上的酶保留的活性水平与自由酶相当。可以认为,关于CeO的性质的本研究的发现2纤维不仅会对抗菌和抗菌试剂领域产生重大影响,而且还将对生物传感器和生物催化剂产生重大影响。

更新日期:2020-06-06
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