Plant height is one of the most important agronomic traits in soybean. The semi-dwarf soybean cultivars could improve lodging resistance and solar radiation use efficiency so as to increase soybean yield. However, the inheritance of semi-dwarf traits has not been intensively studied and few semi-dwarf genes have been isolated in soybean. In this study, we use specific-length amplified fragment sequencing (SLAF-seq) with bulked segregant analysis (BSA) to map the semi-dwarf gene in soybean. Plant height of 213 F 2:3 families, derived from a cross between ‘Jimidou-1’ (semi-dwarf) and ‘Gongjiao9112’ (tall), was evaluated under field conditions. Genetic analysis indicated that the semi-dwarf trait was controlled by one single recessive gene. Associated with the semi-dwarf trait, SLAF-seq combined with BSA was used to develop polymorphic SLAF markers and identify a 1.04 Mb genomic region, which was designated as sdf -1 . The sdf -1 locus was delimited to 80.72 kb region on chromosome 19 by KASP SNP markers developed from the SLAF markers and 6 K SNP chip. There were 14 putative genes in the associated region. From annotation information, three genes ( Glyma.19g194800 , Glyma.19g194500 and Glyma.19g195200 ) might be interesting candidate genes that are highly related to plant height growth or semi-dwarf trait. The successful identification and localization of sdf -1 could help reveal molecular mechanisms underlying soybean semi-dwarfism and to indirectly improve total yield.

中文翻译：

---

SLAF-seq方法鉴定大豆半矮化位点（sdf-1）并进行分子定位

株高是大豆最重要的农艺性状之一。半矮化大豆品种可以提高抗倒性和太阳辐射利用效率，从而提高大豆产量。然而，半矮化性状的遗传尚未得到深入研究，在大豆中分离到的半矮化基因很少。在本研究中，我们使用特定长度扩增片段测序 (SLAF-seq) 和批量分离分析 (BSA) 来定位大豆中的半矮化基因。在田间条件下评估了 213 个 F 2:3 科的株高，这些科源自'Jimidou-1'（半矮）和'Gongjiao9112'（高）之间的杂交。遗传分析表明，半矮化性状由一个隐性基因控制。与半矮人特征相关，SLAF-seq 结合 BSA 用于开发多态性 SLAF 标记并鉴定 1.04 Mb 基因组区域，该区域被指定为 sdf -1 。sdf -1 基因座被从 SLAF 标记和 6 K SNP 芯片开发的 KASP SNP 标记定界到 19 号染色体上的 80.72 kb 区域。在相关区域有 14 个推定基因。从注释信息来看，三个基因（ Glyma.19g194800 、 Glyma.19g194500 和 Glyma.19g195200 ）可能是与株高生长或半矮化性状高度相关的有趣候选基因。sdf -1的成功鉴定和定位有助于揭示大豆半矮化的分子机制，间接提高总产量。由SLAF标记和6 K SNP芯片开发的KASP SNP标记在19号染色体上的72 kb区域。相关区域中有14个推定基因。从注释信息来看，三个基因（ Glyma.19g194800 、 Glyma.19g194500 和 Glyma.19g195200 ）可能是与株高生长或半矮化性状高度相关的有趣候选基因。sdf -1的成功鉴定和定位有助于揭示大豆半矮化的分子机制，间接提高总产量。由SLAF标记和6 K SNP芯片开发的KASP SNP标记在19号染色体上的72 kb区域。相关区域中有14个推定基因。从注释信息来看，三个基因（ Glyma.19g194800 、 Glyma.19g194500 和 Glyma.19g195200 ）可能是与株高生长或半矮化性状高度相关的有趣候选基因。sdf -1 的成功鉴定和定位有助于揭示大豆半矮化的分子机制，间接提高总产量。