The use of high performance thin-layer chromatography (HPTLC) in combination with high resolution time of flight mass spectrometry (MS) for the detection, identification and imaging (HPTLC/MSI) of ecdysteroids (insect moulting hormones) present in a number of plant extracts obtained from members of the Silene family is demonstrated. DESI is shown to be a convenient method for the recovery of these polar polyhydroxylated steroids from the silica gel of the HPTLC plate for subsequent MS detection and imaging. The incorporation of an ion mobility separation (IMS) to the system to give HPTLC/IMS/MS provided additional drift time data which enabled more confident identification. Using HPTLC/DESI/IMS/MS, a range of ecdysteroids were detected and characterized in extracts of S. otitis, S nutans, S. maritime, S. viridiflora and S. fimbriata.

中文翻译：

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植物蜕皮激素的高性能薄层色谱结合解吸电喷雾电离-离子迁移率-飞行时间高分辨率质谱（HPTLC/DESI/IM/ToFMS）

使用高效薄层色谱 (HPTLC) 结合高分辨率飞行时间质谱 (MS) 检测、鉴定和成像 (HPTLC/MSI) 存在于许多植物中的蜕皮激素（昆虫蜕皮激素）展示了从 Silene 家族成员中获得的提取物。DESI 被证明是一种从 HPTLC 板的硅胶中回收这些极性多羟基类固醇的方便方法，用于随后的 MS 检测和成像。将离子淌度分离 (IMS) 纳入系统以提供 HPTLC/IMS/MS 提供额外的漂移时间数据，从而实现更可靠的识别。使用 HPTLC/DESI/IMS/MS，在 S. otitis、S nutans、S. Maritime、S. viridiflora 和 S. fimbriata 的提取物中检测并表征了一系列蜕皮类固醇。