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Effects on development and microbial community of shrimp Litopenaeus vannamei larvae with probiotics treatment.
AMB Express ( IF 3.5 ) Pub Date : 2020-06-05 , DOI: 10.1186/s13568-020-01041-3
Ruixuan Wang 1, 2 , Zihan Guo 3 , Yapeng Tang 1 , Jiawei Kuang 4 , Yafei Duan 1 , Heizhao Lin 1 , Shigui Jiang 1, 5 , Hu Shu 4 , Jianhua Huang 1, 5
Affiliation  

Shrimp production is the second ranked of the most-traded production in these decades and the whiteleg shrimp Litopenaeus vannamei is the sixth most cultured species. Probiotics are alternative strategy for the promotion of growth and prevention of diseases in aquaculture. To confirm the effects of the probiotics on development and microbial community of L. vannamei larvae during different development stages, five kinds of probiotics (108 ~ 109 CFU/g) were added into the rearing environment of shrimp larvae, and the effects of probiotics on bacterial community and water quality, larval growth and immune index were determined from nauplius larval stage to post larval stage. Results suggested that probiotics treated groups showed larger survival rate than the control groups from Z1 stage to P5 stage. Lactobacillus could improve the larvae’s survival ability, especially in the larval stages M2, M3, P1, P5 stage. It was confirmed that probiotics could promote the growth and development of shrimp larvae and prevent the incomplete molting in their growing process, particularly for EM-treated group. Results suggested that all the probiotics-treated groups had shown significant decreasing trend in the quantity of vibrios, except for the SA-treated group. And different probiotics could inhibit vibrios during different life periods. Among these probiotics, LA, EM and PB had shown the best effects, including improving survival rate of the larvae, promoting the larval metamorphosis, reducing the quantity of vibrios and NH4-N and NO2-N levels, and increasing bacterial diversity.



中文翻译:

益生菌处理对虾南美白对虾幼虫发育和微生物群落的影响。

在过去的几十年中,虾产量是交易量最高的产量的第二位,而腿虾南美白对虾是养殖量第六高的物种。益生菌是促进水产养殖生长和预防疾病的替代策略。为了证实在开发和微生物群落益生菌的影响南美白对虾在不同发展阶段的幼虫五种益生菌(10 8  〜10 9 将CFU / g)添加到虾幼体的饲养环境中,并测定了从无节幼体阶段到幼体后期的益生菌对细菌群落和水质,幼虫生长和免疫指数的影响。结果表明,从Z1期到P5期,益生菌治疗组的存活率均高于对照组。乳杆菌可以提高幼虫的存活能力,尤其是在幼虫M2,M3,P1,P5期。证实了益生菌可以促进虾幼虫的生长和发育,并防止其生长过程中的不完全蜕皮,特别是对于EM处理组。结果表明,除SA治疗组外,所有益生菌治疗组的弧菌数量均呈显着下降趋势。不同的益生菌可以在不同的生命周期内抑制弧菌。在这些益生菌中,LA,EM和PB表现出最好的效果,包括提高幼虫的存活率,促进幼虫变态,减少弧菌和NH的数量4 -N和NO 2 -N含量增加,细菌多样性增加。

更新日期:2020-06-05
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