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Laser ablation-ICP-TOFMS imaging of germ cell tumors of patients undergoing platinum-based chemotherapy.
Metallomics ( IF 2.9 ) Pub Date : 2020-06-05 , DOI: 10.1039/d0mt00080a Sarah Theiner 1 , Andreas Schweikert , Christine Haberler , Andreas Peyrl , Gunda Koellensperger
Metallomics ( IF 2.9 ) Pub Date : 2020-06-05 , DOI: 10.1039/d0mt00080a Sarah Theiner 1 , Andreas Schweikert , Christine Haberler , Andreas Peyrl , Gunda Koellensperger
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A low dispersion laser ablation setup in combination with inductively coupled plasma-time-of-flight mass spectrometry (LA-ICP-TOFMS) was applied to clinical samples of patients undergoing platinum-based chemotherapy. The platinum accumulation together with the distribution of elements with biological key functions (Mg, P, S, Ca, Fe, Cu and Zn) was studied in central nervous system germ cell tumor (CNS GCT) tissue, which is an aggressive tumor type located in the brain. Heterogeneous elemental distribution patterns were obtained with a pixel size of 10 μm and were correlated to histological analysis of serial sections using hematoxylin eosin staining. Highest platinum accumulation correlated with areas of necrosis, which exhibited high levels of magnesium, sulphur and calcium. Small traces of gadolinium were found in the tumor sections, which is a result of prior magnetic resonance imaging. Iron accumulated in regions, which were dense in blood vessels, whereas areas with fibrosis scar showed the lowest levels of all detected elements. This LA-ICP-TOFMS study demonstrates that the chemotherapeutic drug cisplatin accumulated in the germ cell tumor located in the brain, which is also reflected by the therapy response of the patients.
中文翻译：
接受铂类化疗患者生殖细胞肿瘤的激光消融-ICP-TOFMS 成像。
将低色散激光消融装置与电感耦合等离子体飞行时间质谱 (LA-ICP-TOFMS) 相结合，应用于接受铂类化疗患者的临床样本。在中枢神经系统生殖细胞肿瘤 (CNS GCT) 组织中研究了铂的积累以及具有生物学关键功能的元素 (Mg、P、S、Ca、Fe、Cu 和 Zn) 的分布，这是一种侵袭性肿瘤类型。在大脑中。获得了像素大小为 10 μm 的异质元素分布模式，并与使用苏木精伊红染色的连续切片的组织学分析相关联。最高的铂积累与坏死区域相关，这些区域表现出高水平的镁、硫和钙。在肿瘤切片中发现了少量钆，这是先前磁共振成像的结果。铁在血管中密集的区域积累，而纤维化疤痕区域显示出所有检测到的元素的最低水平。这项 LA-ICP-TOFMS 研究表明，化疗药物顺铂在位于大脑的生殖细胞肿瘤中积累，这也反映在患者的治疗反应上。
更新日期：2020-06-05
中文翻译：
接受铂类化疗患者生殖细胞肿瘤的激光消融-ICP-TOFMS 成像。
将低色散激光消融装置与电感耦合等离子体飞行时间质谱 (LA-ICP-TOFMS) 相结合，应用于接受铂类化疗患者的临床样本。在中枢神经系统生殖细胞肿瘤 (CNS GCT) 组织中研究了铂的积累以及具有生物学关键功能的元素 (Mg、P、S、Ca、Fe、Cu 和 Zn) 的分布，这是一种侵袭性肿瘤类型。在大脑中。获得了像素大小为 10 μm 的异质元素分布模式，并与使用苏木精伊红染色的连续切片的组织学分析相关联。最高的铂积累与坏死区域相关，这些区域表现出高水平的镁、硫和钙。在肿瘤切片中发现了少量钆，这是先前磁共振成像的结果。铁在血管中密集的区域积累，而纤维化疤痕区域显示出所有检测到的元素的最低水平。这项 LA-ICP-TOFMS 研究表明，化疗药物顺铂在位于大脑的生殖细胞肿瘤中积累，这也反映在患者的治疗反应上。
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