Camels have evolved various resistance characteristics adaptive to their desert habitats. In the present study, we used high-throughput sequencing to investigate stress-induced alternative splicing events as well as different genes involved in resistance to water deprivation and salt absorption in the ileum and liver in Camelus bactrianus. Through association analyses of mRNA, miRNA and lncRNA, we sought to explicate how camels respond to high salt and water scarcity conditions. There were two modes by which genes driven by alternative splicing were enriched to molecular functions, invoking of which was potentially fixed by organ and stress types. With qRT-PCR detection, the differentially expressed MUC6, AQP5, LOC105076960, PKP4, CDH11, TENM1, SDS, LOC105061856, PLIN2 and UPP2 were screened as functionally important genes, along with miR-29b, miR-484, miR-362-5p, miR-96, miR-195, miR-128 and miR-148a. These genes contributed to cellular stress resistance, for instance by reducing water loss, inhibiting excessive import of sodium, improving protective barriers and sodium ion homeostasis, and maintaining uridine content. The underlying competing endogenous RNAs referred to LNC001664, let-7e and LOC105076960 mRNA in ileum, and LNC001438, LNC003417, LNC001770, miR-199c and TENM1 mRNA in liver. Besides competent interpretation to resistance, there may be inspirations for curing human diseases triggered by high-salt intake.

中文翻译：

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回肠和肝转录组对骆驼对高盐和缺水条件的抵抗力的证据。

骆驼已经进化出各种适应其沙漠栖息地的抗性特征。在本研究中，我们使用高通量测序来研究压力诱导的选择性剪接事件，以及参与双峰驼回肠和肝脏中对水分缺乏和盐吸收的抗性的不同基因。通过对mRNA，miRNA和lncRNA的关联分析，我们试图阐明骆驼如何应对高盐和缺水条件。交替剪接驱动的基因有两种模式可以丰富分子功能，而器官和应激类型可能会激活这种功能。通过qRT-PCR检测，将差异表达的MUC6，AQP5，LOC105076960，PKP4，CDH11，TENM1，SDS，LOC105061856，PLIN2和UPP2与miR-29b一起筛选为功能重要的基因，miR-484，miR-362-5p，miR-96，miR-195，miR-128和miR-148a。这些基因例如通过减少水分流失，抑制钠的过量进口，改善保护性屏障和钠离子稳态以及维持尿苷含量而有助于抗细胞应激。潜在的竞争性内源RNA在回肠中称为LNC001664，let-7e和LOC105076960 mRNA，在肝中称为LNC001438，LNC003417，LNC001770，miR-199c和TENM1 mRNA。除了对抗药性有充分的解释，高盐摄入可能会激发治疗人类疾病的灵感。并保持尿苷含量。潜在的竞争性内源RNA在回肠中称为LNC001664，let-7e和LOC105076960 mRNA，在肝中称为LNC001438，LNC003417，LNC001770，miR-199c和TENM1 mRNA。除了对抗药性有充分的解释，高盐摄入可能会激发治疗人类疾病的灵感。并保持尿苷含量。潜在的竞争性内源RNA在回肠中称为LNC001664，let-7e和LOC105076960 mRNA，在肝中称为LNC001438，LNC003417，LNC001770，miR-199c和TENM1 mRNA。除了对抗药性有充分的解释，高盐摄入可能会激发治疗人类疾病的灵感。