Label-free test kit for D-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.,Analytical Methods

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Label-free test kit for D-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-05 , DOI: 10.1039/d0ay00800a
Yan Zeng _{1,

2,

3} , Peng Qi _{1,

2,

3} , Dun Zhang _{1,

2,

3}

Affiliation

The level of D-amino acids (DAAs) is significantly related to bacterial contamination in health and disease, food science and nutrition, and industrial applications. Most sensing methods for the detection of DAAs need expensive equipment, and skilled operation experience, making the test kit for DAA analysis much more complex and challenging. Toward this end, we exploited a label-free DAA test kit based on 1,4-benzenediboronic acid (BDBA)-induced gold nanoparticles (AuNPs) aggregations. DAAs were first catalyzed by their specific catalase (DAAO, D-amino acid oxidase) and oxidized to produce H₂O₂. Then, the produced H₂O₂ inhibited the citrate-capped AuNPs aggregation under BDBA, while the unreacted BDBA could lead to AuNPs aggregation. As a result, the UV/vis absorption spectra and optical photographs of the AuNPs solution are changed in the presence of different DAA target amounts. This method not only can be used for qualitative and semi-quantitative analysis with a naked-eye readout, but also it can quantify DAAs in aqueous solutions with high sensitivity and specificity. Furthermore, the DAAs test kit provided an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples. We believe this DAA test kit provides the potential to be further developed for DAA detection for satisfying both lab and practical needs in different fields.

中文翻译：

通过 1,4-苯二硼酸诱导金纳米粒子聚集进行 D-氨基酸分析的无标记测试套件。

D-氨基酸 (DAA)的水平与健康和疾病、食品科学和营养以及工业应用中的细菌污染显着相关。大多数检测 DAA 的传感方法需要昂贵的设备和熟练的操作经验，这使得用于 DAA 分析的测试套件变得更加复杂和具有挑战性。为此，我们开发了一种基于 1,4-苯二硼酸 (BDBA) 诱导的金纳米粒子 (AuNP) 聚集的无标记 DAA 测试套件。DAA首先被其特定的过氧化氢酶（DAAO， D-氨基酸氧化酶）催化并氧化产生H ₂ O ₂。然后，产生的H ₂ O ₂抑制了BDBA下柠檬酸盐封端的AuNPs聚集，而未反应的BDBA可能导致AuNPs聚集。结果，AuNPs 溶液的紫外/可见吸收光谱和光学照片在不同 DAA 目标量的存在下发生变化。该方法不仅可用于肉眼读数的定性和半定量分析，而且可以高灵敏度和特异性地定量水溶液中的DAA。此外，DAAs 检测试剂盒可有效、选择性地对细菌样品中的 DAA 进行定量，具有出色的精度和准确度。我们相信该DAA检测试剂盒为DAA检测提供了进一步开发的潜力，以满足不同领域的实验室和实际需求。

更新日期：2020-07-09

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