Mapping the normal eye proteome in healthy persons is essential to unravel the molecular basis of diseases impacting visual health. The vitreous occupies a large portion of the human eye between the lens and the retina and plays a significant role in vitreoretinal diseases as well as maintaining clarity in the visual field, providing nutrition to the lens, and protecting the eye from mechanical shocks. It comprises four distinct anatomical regions, namely the vitreous core, vitreous cortex, vitreous base, and anterior hyaloid. Among these, the vitreous is attached to other substructures in the eye by the vitreous base, which is its strongest point of attachment. Alterations in vitreous substructures have been reported in several vitreoretinal disorders, including vitreomacular traction, vitreoretinopathies, and age-related macular degeneration. There has been limited knowledge on proteomics variations at a resolution of vitreous substructures, including the functionally and pathophysiologically significant vitreous base. We report here new findings on the proteome map of the vitreous base in normal healthy tissue. We employed a global, unbiased proteomic profiling approach resulting in the identification of 6511 proteins. Of these, 302 proteins were involved in metabolic processes essential for energy utilization. Moreover, we identified several structural and nutrient transport proteins. Notably, the identified proteome repertoire indicates that the vitreous base might possess additional physiological functions and may not be a passive structure. This study constitutes the most extensive catalog of vitreous base proteins to our knowledge and offers novel insights as a baseline for future studies on the pathobiology of various eye diseases. These data also invite us to consider a potentially more active functional role for the vitreous base in eye physiology and visual health.

中文翻译：

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在玻璃亚结构中深入研究眼部蛋白质组：正常人玻璃体基础的高分辨率蛋白质组图。

绘制健康人的正常眼部蛋白质组图对于揭示影响视力健康的疾病的分子基础至关重要。玻璃体在晶状体和视网膜之间占据了人眼的很大一部分，并且在玻璃体视网膜疾病以及维持视野透明性，为晶状体提供营养并保护眼睛免受机械冲击方面起着重要作用。它包括四个不同的解剖区域，即玻璃体核心，玻璃体皮质，玻璃体基底和前玻璃体。其中，玻璃体通过玻璃体基底附着在眼睛的其他子结构上，这是其最强的附着点。在几种玻璃体视网膜疾病中已报道玻璃体亚结构的改变，包括玻璃体牵引，玻璃体视网膜病变和与年龄有关的黄斑变性。关于蛋白质组学变异的知识在玻璃亚结构的分辨率方面是有限的，包括功能上和病理生理上重要的玻璃基。我们在这里在正常健康组织的玻璃体基质的蛋白质组图上报告新发现。我们采用了一种全球公正的蛋白质组学分析方法，从而鉴定了6511种蛋白质。其中，302种蛋白质参与了能量利用必不可少的代谢过程。此外，我们鉴定了几种结构和营养转运蛋白。值得注意的是，已鉴定的蛋白质组库表明玻璃体碱基可能具有其他生理功能，并且可能不是被动结构。这项研究构成了我们所掌握的最广泛的玻璃体基础蛋白目录，并提供了新颖的见解，作为今后各种眼病病理生物学研究的基础。这些数据还邀请我们考虑在眼部生理和视觉健康方面，潜在的更活跃的玻璃体功能角色。