Titanium (Ti) is widely used in oral implants. However, there is still a challenge to promote the osseointegration of bone tissue on the surface of Ti. To solve this problem, we prepared novel gradient nanostructured (GNS) Ti and studied the effect of GNS on the adhesion, proliferation, and apoptosis of MG63 cells in vitro. The results demonstrated that the GNS Ti promoted the adhesion effect and proliferation of MG63 cells better than the annealed Ti, while the ability of GNS Ti to inhibit cell apoptosis was better than that of the annealed Ti, the preliminary mechanism of which indicated by this study might be the enhanced mineralization capacity, protein adsorption ability, and hydrophilicity of the GNS Ti due to its specific nanostructure which improved the cell behaviours. The results in this study provide the theoretical and experimental foundations for the applications of GNS Ti in dental implants and joint replacements.

中文翻译：

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梯度纳米结构Ti对MG63细胞体外行为的影响

钛（Ti）广泛用于口腔植入物。然而，仍然存在促进钛表面上骨组织的骨整合的挑战。为了解决这个问题，我们制备了新型梯度纳米结构（GNS）Ti，并研究了GNS对MG63细胞粘附，增殖和凋亡的影响。。结果表明，GNS Ti比退火的Ti具有更好的促进MG63细胞的黏附作用和增殖，而GNS Ti抑制细胞凋亡的能力优于退火的Ti，其初步机制提示可能是由于GNS Ti的特定纳米结构改善了细胞的行为，使其矿化能力，蛋白质吸附能力和亲水性增强。这项研究的结果为GNS Ti在牙种植体和关节置换中的应用提供了理论和实验基础。