Electrospun fiber membranes have been extensively researched for tissue repair; however, fiber angiogenesis has been a difficult problem because the biomaterials used to prepare the fiber cannot promote angiogenesis by themselves. To endow the fiber with the function of promoting angiogenesis and oral mucosa regeneration, surface-aminated liposomes (NH₂-LIPs) encapsulating leptin, as well as silk fiber (SF) membranes that have been immersed overnight in polydopamine (PDA) solution were synthesized. The NH₂-LIPs were then grafted onto the surfaces of SF through reactions between the catechol groups of PDA and the amino groups of NH₂-LIPs to induce angiogenesis on the fiber surface and thus promote oral mucosa regeneration. The PDA-modified and NH₂-LIP-modified SF retained the original fibrous morphology but increased the SF tensile strength from 1.95 MPa to 2.87 MPa. PDA changed the hydrophilicity of the fibers and improved the adhesion of fibroblasts on the fibrous membranes. Leptin loaded onto SF via PDA had no significant effect on cell proliferation and formed 162.7-node tubes in human umbilical vein endothelial cells (HUVECs) within 10 h, which demonstrated that leptin loaded onto SF can promote angiogenesis. Wound closure of mucosa covered in leptin-loaded fibrous membranes reached 99% at 14 days postsurgery. Histologic analysis showed that leptin-loaded SFs exhibited clear stratification of new mucosa, as well as a strong CD34 signal that indicated the presence of new blood vessels and confirmed the successful loading of leptin on the fiber. Therefore, this work successfully showed that NH₂-LIPs grafted onto the surface of SFs via PDA endow the fibers with angiogenic abilities and promote oral mucosa regeneration.

电纺纤维膜已被广泛研究用于组织修复。然而，由于用于制备纤维的生物材料本身不能促进血管生成，因此纤维血管生成一直是一个难题。为了使纤维具有促进血管生成和口腔粘膜再生的功能，合成了包裹瘦蛋白的表面胺化脂质体（NH ₂ -LIPs）以及已浸入聚多巴胺（PDA）溶液中过夜的丝纤维（SF）膜。 。然后通过PDA的儿茶酚基团和NH ₂ -LIPs的氨基之间的反应将NH ₂ -LIPs接枝到SF的表面上，以诱导纤维表面上的血管生成，从而促进口腔粘膜再生。PDA改性和NH ₂-LIP改性的SF保留了原始的纤维形态，但将SF的拉伸强度从1.95 MPa增加到2.87 MPa。PDA改变了纤维的亲水性，并改善了成纤维细胞在纤维膜上的附着力。通过PDA加载到SF上的瘦素对细胞增殖没有明显影响，并在10 h内在人脐静脉内皮细胞（HUVEC）中形成了162.7个节点的管，这表明，加载到SF上的瘦素可以促进血管生成。术后14天，覆盖有瘦素的纤维膜覆盖的粘膜伤口闭合率达到99％。组织学分析表明，载有瘦素的SF表现出清晰的新粘膜分层，以及强烈的CD34信号，表明存在新血管，并证实瘦素已成功载入纤维。因此，通过PDA移植到SFs表面的_2- LIPs使纤维具有血管生成能力并促进口腔粘膜再生。