Abstract

Propionate has antimicrobial activity and is suggested to influence lipid metabolism. Here, we investigated the effect of propionate on lipid metabolism and the gut microbiome in fructose-fed mice as a model of diet-induced steatosis and gut dysbiosis. Therefore, 48 male wild-type mice were fed isoenergetic diets with either 0% fructose (F–) or 40% fructose (F+) that contained 0% propionate (P–) or 1% propionate (P+) for 7 weeks. Mice that received the F+ diets developed fatty livers, had fewer small intestinal proteobacteria and colonic actinobacteria and were characterised by changes in bacterial genera (e.g., Allobaculum, Lachnospiraceae, and Escherichia). Interestingly, mice fed the F+ diets had higher levels of propionate and butyrate in the circulation than mice fed the F– diets (p < 0.05). Treatment with propionate influenced neither hepatic or plasma lipids nor levels of circulating SCFAs. With the exception of Verrucomicrobia, other bacterial phyla were not affected by propionate.

摘要

丙酸盐具有抗菌活性，并被建议影响脂质代谢。在这里，我们研究了丙酸盐对果糖喂养小鼠脂质代谢和肠道微生物组的影响，作为饮食诱导的脂肪变性和肠道菌群失调的模型。因此，48 只雄性野生型小鼠被喂食含有 0% 果糖 (F-) 或 40% 果糖 (F+) 且含有 0% 丙酸盐 (P-) 或 1% 丙酸盐 (P+) 的等能饮食 7 周。接受 F+ 饮食的小鼠出现脂肪肝，小肠变形菌和结肠放线菌较少，并以细菌属（如异芽孢杆菌、毛螺菌科和埃希氏菌）的变化为特征。）。有趣的是，喂食 F+ 饮食的小鼠在循环中的丙酸盐和丁酸盐水平高于喂食 F- 饮食的小鼠（p  < 0.05）。丙酸盐治疗既不影响肝脏或血浆脂质，也不影响循环 SCFA 的水平。除疣微菌外，其他细菌门不受丙酸盐影响。