Abstract

The aims of this study were to analyze the effects of PPARGC1B and ZEB2 polymorphisms on the body weight of Hu sheep. DNA sequencing and KASPar technologies were used to detect single nucleotide polymorphisms (SNPs) within the PPARGC1B and ZEB2 genes of Hu sheep (n = 207). Two synonymous mutations, g.300 G > A and g.645 C > T, were detected in PPARGC1B and ZEB2, respectively. The body weights of sheep were recorded at 80, 100, 120, 140, 160 and 180-days, and associations between these polymorphisms and body weight changes were analyzed. Association analysis demonstrated that the polymorphisms in PPARGC1B and ZEB2 significantly associated with body weight (p < 0.05). At the g.300 G > A locus, individuals with the GG genotype had significantly higher body weight than those with the AA genotype, and at the g.645 C > T locus, individuals with the TT genotype had significantly higher body weight than those with the TC genotype. Individuals with both polymorphisms exhibited significantly different growth (p < 0.05). These data suggest that polymorphisms in the PPARGC1B and ZEB2 genes can be used as candidate molecular markers for the breeding of desirable growth traits in Hu sheep.

摘要

本研究旨在分析PPARGC1B和ZEB2多态性对胡羊体重的影响。DNA测序和KASPar技术用于检测胡羊（n  = 207）PPARGC1B和ZEB2基因内的单核苷酸多态性（SNP）。在PPARGC1B和ZEB2中分别检测到两个同义突变 g.300 G > A 和 g.645 C > T。在80、100、120、140、160和180天记录绵羊的体重，并分析这些多态性与体重变化之间的关联。关联分析表明PPARGC1B和ZEB2的多态性与体重显着相关（p  < 0.05）。在g.300 G > A位点，GG基因型个体的体重显着高于AA基因型个体，而在g.645 C > T位点，TT基因型个体的体重显着高于那些基因型个体。与 TC 基因型。具有两种多态性的个体表现出显着不同的生长（p  < 0.05）。这些数据表明，PPARGC1B和ZEB2基因的多态性可以作为候选分子标记，用于育种胡羊所需的生长性状。