ABSTRACT

An LC-MS/MS multi-residue method was validated for the determination of 59 pesticide residue levels in a chicken egg by using QuEChERS extraction method. The validation parameters used were taken from the SANTE/11813/2017 guideline. The calibration curves for each target pesticide had a good linearity in a range of 1.0 or 2.5 to 100.0 µg kg⁻¹ (r² ≥ 0.995). Ten per cent of the total pesticides had LOQ values less than 5.0 µg kg⁻¹ or LOQ values from 5.0 to 10.0 µg kg⁻¹. The LOQ values were below the MRLs set by the European Commision (EC). Average recoveries (70–120%) and relative standard deviations (RSDs≤20%) were achieved for 59 selected pesticides at different concentrations (5 or 10 and 200 µg kg⁻¹). All expanded measurement uncertainties were lower than 50%. The occurrence of these pesticides was evaluated in 35 commercially available chicken eggs. No MRL exceedance was identified.

摘要

通过QuEChERS提取方法验证了LC-MS / MS多残留方法用于测定鸡蛋中59种农药残留水平。使用的验证参数取自SANTE / 11813/2017指南。针对每个目标的农药校准曲线具有范围为1.0或2.5的良好的线性度到100.0微克千克^-1（R ²  ≥0.995）。农药总量的10％的LOQ值小于5.0 µg kg ^-1或LOQ值从5.0至10.0 µg kg ^-1。LOQ值低于欧洲委员会（EC）设定的最大残留限量。选择59种不同浓度（5或10和200 µg kg ^-1的农药）的平均回收率（70–120％）和相对标准偏差（RSDs≤20％）。）。所有扩展的测量不确定度均低于50％。在35种市售鸡蛋中评估了这些农药的存在。没有发现最大残留限量。