Introduction

Bioactive molecule carrier systems (BACS) are biomaterial-based substrates that facilitate the delivery of active signaling molecules for different biologically based therapeutic applications, which include regenerative endodontic procedures. Tissue regeneration or organized repair in regenerative endodontic procedures is governed by the dynamic orchestration of interactions between stem/progenitor cells, bioactive molecules, and extracellular matrix. BACS aid in mimicking some of the complex physiological processes, overcoming some of the challenges faced in the clinical translation of regenerative endodontic procedures.

Areas covered

This narrative review addresses the role of BACS in stem/progenitor cell proliferation, migration, and differentiation with the application for dentin-pulp tissue engineering both in vitro and in vivo. BACS shield the bioactivity of the immobilized molecules against environmental factors, while its design allows the pre-programmed release of bioactive molecules in a spatial and temporal-controlled manner. The polymeric and non-polymeric materials used to synthesize micro and nanoscale-based BACS are reviewed.

Expert opinion

Comprehensive characterization of well-designed and customized BACS is necessary to be able to deliver multiple bioactive molecules in spatiotemporally controlled manner and to address the release kinetics required for potential in vivo application. This warrants further laboratory-based experiments and rigorous clinical investigations to enable their clinical translation for regenerative endodontic procedures.

中文翻译：

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牙髓中的生物活性分子载体系统。

介绍

生物活性分子载体系统（BACS）是基于生物材料的基质，可促进递送活性信号分子，以用于不同的基于生物学的治疗应用，包括再生牙髓治疗程序。再生牙髓手术过程中的组织再生或有组织的修复受干/祖细胞，生物活性分子和细胞外基质之间相互作用的动态协调控制。BACS有助于模仿一些复杂的生理过程，克服了再生牙髓手术的临床翻译中面临的一些挑战。

覆盖区域

这种叙述审查地址BACS的干/祖细胞的增殖，迁移和分化中的作用与两个牙本质牙髓组织工程中的应用在体外和体内。BACS保护固定分子对环境因素的生物活性，而BACS的设计则允许以空间和时间控制的方式预先编程释放生物活性分子。综述了用于合成微米级和纳米级BACS的聚合材料和非聚合材料。

专家意见

为了能够以时空受控方式递送多个生物活性分子并解决潜在体内应用所需的释放动力学，必须对精心设计和定制的BACS进行全面表征。这保证了进一步的基于实验室的实验和严格的临床研究，以使其可用于再生牙髓治疗程序的临床翻译。