Here, we prepared the novel combined adjuvants, CTB as intra-molecular adjuvant, CpG and aluminum hydroxide (Alum) to strengthen the immunogenicity of clumping factor A_221-550 of Staphylococcus aureus (S. aureus). The protein-immunoactive results showed CTB-ClfA_221-550 elicited the strong immune responses to serum from mice immunized with CTB and ClfA_221-550, respectively. The mice immunized with CTB-ClfA_221-550 plus CpG and Alum adjuvant exhibited significantly stronger CD4⁺ T cell responses for IFN-γ, IL-2, IL-4, and IL-17 and displayed the higher proliferation response of splenic lymphocytes than the control groups, in addition, these mice generated the strongest humoral immune response against ClfA_221-550 among all groups. Our results also showed CTB-ClfA_221-550 plus CpG and Alum adjuvant obviously increased the survival percentage of the mice challenged by S. aureus. These data suggested that the novel combined adjuvants, CTB, CpG, and Alum, significantly enhance the immune responses triggered with ClfA_221-550, and could provide a new approach against infection of S. aureus.

Abbreviations

CTB: Cholera Toxin B; CpG: Cytosine preceding Guanosine; ODN: Oligodeoxynucleotides; Alum: Aluminum hydroxide; TRAP: Target of RNAIII-activating Protein; TLR9: Toll-like Receptor 9; TMB: 3, 3ʹ, 5, 5ʹ-tetramethylbenzidine; mAbs: Monoclonal Antibodies; OD: Optical Densities; S. aureus: Staphylococcus aureus; ClfA: Clumping factor A; FnBPA: Fibronection-binding protein A; IsdB: Iron-regulated surface determinant B; SasA: Staphylococcus aureus Surface Protein A; GapC: Glycer-aldehyde-3-phosphate dehydrogenase-C

中文翻译：

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CTB，CpG和氢氧化铝的新型组合显着增强了金黄色葡萄球菌的聚集因子A 221-550的免疫原性。

在这里，我们制备的新组合佐剂，如CTB分子内佐剂，CpG和氢氧化铝（明矾），以加强聚集因子A的免疫原性_221-550的金黄色葡萄球菌（金黄色葡萄球菌）。蛋白质免疫活性结果表明，CTB-ClfA _{221-550分别}引起了用CTB和ClfA _221-550免疫的小鼠对血清的强烈免疫反应。用CTB-ClfA _221-550加CpG和明矾佐剂免疫的小鼠表现出明显更强的CD4 ⁺T细胞对IFN-γ，IL-2，IL-4和IL-17的应答，并显示出脾淋巴细胞的增殖应答高于对照组，此外，这些小鼠产生了针对ClfA _221-550的最强体液免疫应答在所有群体中。我们的结果还表明，CTB-ClfA _221-550加上CpG和明矾佐剂明显提高了金黄色葡萄球菌攻击的小鼠的存活率。这些数据表明，新型组合佐剂CTB，CpG和明矾可显着增强ClfA _221-550触发的免疫反应，并可能提供抗金黄色葡萄球菌感染的新方法。

缩略语

CTB：霍乱毒素B；CpG：鸟苷之前的胞嘧啶；ODN：寡脱氧核苷酸；明矾：氢氧化铝; TRAP：RNAIII激活蛋白的靶标；TLR9：收费型受体9；TMB：3、3ʹ，5、5ʹ-四甲基联苯胺；mAb：单克隆抗体；单克隆抗体 OD：光密度；金黄色葡萄球菌：金黄色葡萄球菌；ClfA：聚集因子A；FnBPA：纤连蛋白A；IsdB：铁调节的表面行列式B；SasA：金黄色葡萄球菌表面蛋白A；GapC：甘油醛-3-磷酸脱氢酶-C