Resistance to carbapenems is a severe threat to human health. These last resort antimicrobials are indispensable for the treatment of severe human infections with multidrug-resistant Gram-negative bacteria. In accordance with their increasing medical impact, carbapenemase-producing Enterobacteriaceae (CPE) might be disseminated from colonized humans to non-human reservoirs (i.e., environment, animals, food). In Germany, the occurrence of CPE in livestock and food has been systematically monitored since 2016. In the 2019 monitoring, an OXA-48-producing E. coli (19-AB01443) was recovered from a fecal sample of a fattening pig. Phenotypic resistance was confirmed by broth microdilution and further characterized by PFGE, conjugation, and combined short-/long-read whole genome sequencing. This is the first detection of this resistance determinant in samples from German meat production. Molecular characterization and whole-genome sequencing revealed that the bla_OXA-48 gene was located on a common pOXA-48 plasmid-prototype. This plasmid-type seems to be globally distributed among various bacterial species, but it was frequently associated with clinical Klebsiella spp. isolates. Currently, the route of introduction of this plasmid/isolate combination into the German pig production is unknown. We speculate that due to its strong correlation with human isolates a transmission from humans to livestock has occurred.

中文翻译：

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从公共卫生溢出？在德国养猪场首次检测到生产OXA-48的大肠杆菌。

对碳青霉烯类药物的抗性是对人类健康的严重威胁。这些最后使用的抗菌剂对于治疗具有多重耐药性的革兰氏阴性细菌的严重人类感染是必不可少的。根据其日益增加的医学影响，产碳青霉烯酶的肠杆菌科（CPE）可能会从定居的人类传播到非人类的水库（即环境，动物，食物）。在德国，CPE的牲畜和食物发生了系统2016，因为监测在监测2019，一个OXA-48生产E.大肠杆菌 从肥育猪的粪便样品中回收（19-AB01443）。表型抗性通过肉汤微量稀释得到确认，并通过PFGE，结合和短/长读全基因组测序进一步表征。这是在德国肉类生产样品中首次检测到这种抗性决定子。分子表征和全基因组测序表明，bla _OXA-48基因位于常见的pOXA-48质粒原型上。这种质粒类型似乎在各种细菌中广泛分布，但通常与临床克雷伯菌有关spp。隔离株。目前，尚不清楚将该质粒/分离物组合引入德国养猪生产的途径。我们推测，由于其与人类分离株的密切相关，已经发生了从人到牲畜的传播。