Green gram, Vigna radiata (L.) Wilczek, is an important pulse crop of Asia. Severe attack by the larvae of Spilosoma obliqua Walker (Lepidoptera: Arctiidae) causes defoliation of green gram and reduces seed yield. Females lay eggs on the leaf surface, and therefore, surface wax plays an important role as short‐range attractant and oviposition stimulant. So, we have attempted to find out whether leaf surface wax compounds (alkanes and free fatty acids) from three green gram cultivars (PDM 54, PUSA BAISAKHI and SAMRAT) could act as short‐range attractant and oviposition stimulant in females. The TLC, GC‐MS and GC‐FID analyses of n‐hexane extracts revealed 20 n‐alkanes from n‐C₁₅ to n‐C₃₆ and 13 free fatty acids from C12:0 to C21:0, whilst linoleic acid was unique in SAMRAT. Pentacosane was the predominant amongst n‐alkanes in the leaf surface waxes of three cultivars. Heneicosanoic acid and palmitoleic acid were the predominant free fatty acids in the leaf surface waxes of PDM 54, and PUSA BAISAKHI and SAMRAT, respectively. Females were attracted towards one leaf equivalent surface wax of three green gram cultivars against solvent controls (n‐hexane) in Y‐tube olfactometer bioassays. A synthetic blend of pentacosane, heptacosane, nonacosane, hexatriacontane, palmitoleic acid, linolenic acid and stearic acid, a synthetic blend of pentacosane, hexatriacontane and stearic acid, and a synthetic blend of hexatriacontane, linolenic acid and stearic acid resembling in amounts present in one leaf equivalent surface wax of PDM 54, PUSA BAISAKHI and SAMRAT, respectively, served as short‐range attractant and oviposition stimulant in females. Females showed equal preference for egg laying towards the above three synthetic blends when these blends were tested against each other, and hence, these blends could be employed in development of baited traps in pest management strategies.

中文翻译：

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叶表面蜡作为全鳞翅目短程引诱剂和产卵刺激物的重要性

绿豆Vigna radiata（L.）Wilczek是亚洲重要的豆类作物。斜长螺旋螺幼虫（鳞翅目：弓形纲）的幼虫的严重侵袭导致绿色克的脱叶并降低种子产量。雌虫在叶片表面产卵，因此表面蜡起着短距离引诱剂和产卵刺激剂的作用。因此，我们试图找出来自三个绿色革兰品种（PDM 54，PUSA BAISAKHI和SAMRAT）的叶片表面蜡化合物（烷烃和游离脂肪酸）是否可以作为雌性的短程引诱剂和产卵刺激剂。的TLC，GC-MS和GC-FID分析ñ -己烷提取物显示20 ñ -烷烃从Ñ -C ₁₅到从C12：0到C21：0的n C ₃₆和13个游离脂肪酸，而亚油酸在SAMRAT中是独特的。在三个品种的叶片表面蜡中，正二十烷是正构烷烃中的主要成分。二十烷酸和棕榈油酸分别是PDM 54和PUSA BAISAKHI和SAMRAT叶片表面蜡中的主要游离脂肪酸。将雌性吸引到三个绿克品种的一叶当量表面蜡上，以防溶剂控制（n-己烷）在Y型管嗅仪生物测定中。戊三烷，庚烷，壬二烷，十六碳三烷，棕榈油酸，亚麻酸和硬脂酸的合成混合物，戊三烷，六三二十烷和硬脂酸的合成混合物，以及六金刚烷，亚麻酸和硬脂酸的合成混合物，其含量为一个PDM 54，PUSA BAISAKHI和SAMRAT的叶片等效表面蜡分别用作雌性的短程引诱剂和产卵刺激剂。当将上述三种混合饲料进行相互测试时，雌性对上述三种混合饲料表现出同等的偏好，因此，这些混合饲料可用于有害生物管理策略中诱饵诱集装置的开发。