The microbial threat: Can rare earths help?,Journal of Biophotonics

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The microbial threat: Can rare earths help?
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-06-22 , DOI: 10.1002/jbio.202000068
Marina M Lezhnina _{1,

2} , Weronika Rochowiak ₂ , Wolfgang Göhde ₁ , Rauni Kuczius ₃ , Ulrich Kynast ₂

Affiliation

Despite an ever increasing demand for reliable and cheap methods in the detection and quantification of microbes, surprisingly few investigations have explored or utilized the luminescence of rare earths in the microbial context, neither in conventional, that is, plating and microscopic imaging techniques, nor in advanced methods like fluorescence flow cytometry. We have thus investigated the potential of some rare earth complexes and hybrid materials for microbiological analysis. We found fairly simple procedures for internal staining (dyes inside the bacterial cell) and external staining (dyes on the cell surface). The present paper is predominantly relying on microscopic imaging and luminescence spectroscopies (excitation, emission, decay times), but also evaluates model rare earth microspheres to estimate an eventual rare earth based stain for a fast and sensitive bacteria enumeration with luminescence flow cytometry.

中文翻译：

微生物威胁：稀土能提供帮助吗？

尽管对微生物检测和定量的可靠且便宜的方法的需求不断增长，但令人惊讶的是，很少有研究在微生物的背景下探索或利用稀土的发光，这既没有常规的方法，即平板和显微镜成像技术，也没有。先进的方法，例如荧光流式细胞术。因此，我们研究了一些稀土配合物和杂化材料在微生物分析中的潜力。我们发现了内部染色（细菌细胞内部的染料）和外部染色（细胞表面的染料）相当简单的程序。本文主要依靠显微镜成像和发光光谱学（激发，发射，衰减时间），

更新日期：2020-06-22

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