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Metabolic regulation of polyamines and γ-aminobutyric acid in relation to spermidine-induced heat tolerance in white clover.
Plant Biology ( IF 4.2 ) Pub Date : 2020-06-05 , DOI: 10.1111/plb.13139 L Luo 1 , Z Li 1 , M Y Tang 1 , B Z Cheng 1 , W H Zeng 1 , Y Peng 1 , G Nie 1 , X Q Zhang 1
中文翻译：
多胺和γ-氨基丁酸的代谢调节与亚精胺诱导的白三叶草耐热性相关。
更新日期：2020-06-05
Plant Biology ( IF 4.2 ) Pub Date : 2020-06-05 , DOI: 10.1111/plb.13139 L Luo 1 , Z Li 1 , M Y Tang 1 , B Z Cheng 1 , W H Zeng 1 , Y Peng 1 , G Nie 1 , X Q Zhang 1
Affiliation
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- Heat stress decreases crop growth and yield worldwide. Spermidine (Spd) is a small aliphatic amine and acts as a ubiquitous regulator for plant growth, development and stress tolerance.
- Objectives of this study were to determine effects of exogenous Spd on changes in endogenous polyamine (PA) and γ‐aminobutyric acid (GABA) metabolism, oxidative damage, senescence and heat shock protein (HSP) expression in white clover subjected to heat stress. Physiological and molecular methods, including colorimetric assay, high performance liquid chromatography and qRT‐PCR, were applied.
- Results showed that exogenous Spd significantly alleviated heat‐induced stress damage. Application of Spd not only increased endogenous putrescine, Spd, spermine and total PA accumulation, but also accelerated PA oxidation and improved glutamic acid decarboxylase activity, leading to GABA accumulation in leaves under heat stress. The Spd-pretreated white clover maintained a significantly higher chlorophyll (Chl) content than untreated plants under heat stress, which could be related to the roles of Spd in up‐regulating genes encoding Chl synthesis (PBGD and Mg‐CHT) and maintaining reduced Chl degradation (PaO and CHLASE) during heat stress. In addition, Spd up‐regulated HSP70, HSP70B and HSP70‐5 expression, which might function in stabilizing denatured proteins and helping proteins to folding correctly in white clover under high temperature stress.
- In summary, exogenous Spd treatment improves the heat tolerance of white clover by altering endogenous PA and GABA content and metabolism, enhancing the antioxidant system and HSP expression and slowing leaf senescence related to an increase in Chl biosynthesis and a decrease in Chl degradation during heat stress.
中文翻译：
多胺和γ-氨基丁酸的代谢调节与亚精胺诱导的白三叶草耐热性相关。
- 热应激会降低全球的作物生长和单产。亚精胺（Spd）是一种小的脂肪胺，可作为植物生长，发育和胁迫耐受性的普遍调节剂。
- 这项研究的目的是确定外源Spd对受热胁迫的三叶草内源多胺（PA）和γ-氨基丁酸（GABA）代谢，氧化损伤，衰老和热休克蛋白（HSP）表达变化的影响。应用了生理和分子方法，包括比色测定，高效液相色谱和qRT-PCR。
- 结果表明，外源Spd显着减轻了热诱导的应力破坏。Spd的施用不仅增加了内源性腐胺，Spd，精胺和总PA的积累，而且还加速了PA的氧化并改善了谷氨酸脱羧酶的活性，导致热胁迫下叶片中GABA的积累。Spd预处理的白三叶草在热胁迫下的叶绿素（Chl）含量比未处理的植物高得多，这可能与Spd在上调Chl合成基因（PBGD和Mg-CHT）并保持Chl减少中的作用有关在热应力下会降解（PaO和CHLASE）。此外，Spd上调了HSP70，HSP70B和HSP70-5表达，这可能会在稳定的蛋白质变性，并帮助蛋白质在高温应力下在白三叶草正确折叠的功能。
- 总之，外源Spd处理通过改变内源PA和GABA含量和代谢，增强抗氧化系统和HSP表达并减缓与衰老有关的Chl生物合成和减少Chl降解相关的叶片衰老，从而提高了白三叶草的耐热性。 。
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