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Graphenic Aerogels Decorated with Ag Nanoparticles as 3D SERS Substrates for Biosensing
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-06-05 , DOI: 10.1002/ppsc.202000095 Glenda Biasotto 1 , Alessandro Chiadò 2 , Chiara Novara 2 , Marco Fontana 3 , Marco Armandi 2 , Maria Aparecida Zaghete 1 , Fabrizio Giorgis 2 , Paola Rivolo 2
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2020-06-05 , DOI: 10.1002/ppsc.202000095 Glenda Biasotto 1 , Alessandro Chiadò 2 , Chiara Novara 2 , Marco Fontana 3 , Marco Armandi 2 , Maria Aparecida Zaghete 1 , Fabrizio Giorgis 2 , Paola Rivolo 2
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A versatile and efficient surface‐enhanced Raman scattering (SERS) substrate based on a hybrid aerogel composed of reduced graphene oxide (rGO) decorated with silver nanoparticles (AgNPs), suitable for highly sensitive label‐free detection of chemical and biological species, is presented. The simple and low‐cost one‐pot hydrothermal synthesis allows obtaining of a 3D nanostructured spongy‐like matrix that shows good spatial distribution of Ag nanoparticles in intimate contact with rGO flakes, characterized by means of several morphological, structural, and compositional techniques. The nanostructured material, tested by SERS analysis with both rhodamine 6G (R6G) and 4‐mercaptobenzoic acid (MBA), shows a satisfying SERS efficiency, quantified in terms of minimum detectable concentration of 10−10 and 10−7 m, corresponding to on‐ and off‐resonant excitation, respectively. The versatility of chemical/biochemical functionalization is successfully demonstrated by exploiting different routes, by immobilizing both protoporphyrin IX (PRPIX) and hemin (H) that take advantage of π−π non‐covalent bonding with the graphene layers, as well as thiol‐ended oligonucleotides (DNA probes/aptamers) directly grafted on the AgNPs. Finally, after the successful integration of the hybrid aerogel into a microfluidic chip, the biorecognition of miR222 is obtained demonstrating the reliability of the aerogel substrate as SERS platform for biosensing.
中文翻译：
Ag纳米颗粒修饰的Graphenic气凝胶作为3D SERS底物用于生物传感
提出了一种多功能，高效的表面增强拉曼散射（SERS）基底，该基底基于混合有还原气的氧化石墨烯（rGO）和银纳米颗粒（AgNPs）构成的混合气凝胶，适用于化学和生物物种的高灵敏度无标记检测。 。简单且低成本的一锅水热合成可以得到3D纳米结构的海绵状基质，该基质在与rGO薄片紧密接触时显示出Ag纳米颗粒的良好空间分布，其特征在于几种形态学，结构和组成技术。通过罗丹明6G（R6G）和4-巯基苯甲酸（MBA）的SERS分析测试的纳米结构材料显示出令人满意的SERS效率，以最低可检测浓度10 -10和10进行了量化-7 m，分别对应于开启和关闭共振。化学/生化功能化的多功能性是通过利用不同的途径成功地证明的，方法是固定化原卟啉IX（PRPIX）和血红素（H），它们利用与石墨烯层以及硫醇封端的π-π非共价键直接嫁接到AgNP上的寡核苷酸（DNA探针/适体）。最终，在将混合气凝胶成功整合到微流控芯片中之后，获得了miR222的生物识别，这证明了气凝胶基质作为SERS平台进行生物传感的可靠性。
更新日期：2020-06-05
中文翻译：
Ag纳米颗粒修饰的Graphenic气凝胶作为3D SERS底物用于生物传感
提出了一种多功能，高效的表面增强拉曼散射（SERS）基底，该基底基于混合有还原气的氧化石墨烯（rGO）和银纳米颗粒（AgNPs）构成的混合气凝胶，适用于化学和生物物种的高灵敏度无标记检测。 。简单且低成本的一锅水热合成可以得到3D纳米结构的海绵状基质，该基质在与rGO薄片紧密接触时显示出Ag纳米颗粒的良好空间分布，其特征在于几种形态学，结构和组成技术。通过罗丹明6G（R6G）和4-巯基苯甲酸（MBA）的SERS分析测试的纳米结构材料显示出令人满意的SERS效率，以最低可检测浓度10 -10和10进行了量化-7 m，分别对应于开启和关闭共振。化学/生化功能化的多功能性是通过利用不同的途径成功地证明的，方法是固定化原卟啉IX（PRPIX）和血红素（H），它们利用与石墨烯层以及硫醇封端的π-π非共价键直接嫁接到AgNP上的寡核苷酸（DNA探针/适体）。最终，在将混合气凝胶成功整合到微流控芯片中之后，获得了miR222的生物识别，这证明了气凝胶基质作为SERS平台进行生物传感的可靠性。
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