On this basis, a novel recombinant human‐like collagen (RHLC)/silk fibroin scaffold material with high porosity and controllable aperture was prepared. The compatibility of osteoblasts (OB) with the blends was tested in vitro. The morphology, adhesion and growth of scaffold cells were observed by scanning electron microscope and laser confocal microscope. Extensive measurements, including 3‐[4, 5‐dimethylthiazole‐2‐acyl]‐2, 5‐diphenyl tetrabrominate assays, intracellular total protein content, and alkaline phosphatase activity assays were performed after 7 days of culture. Survival and protein content increased in RHLC/fibroin stents. LSCM and SEM results confirmed that the cells grew better in the mixed scaffolds than in the pure silk scaffolds, and showed that the cells were easy to adhere and diffuse in the RHLC/silk scaffolds. RHLC/silk fibroin scaffolds are promising biomaterials for bone tissue engineering.

中文翻译：

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制备重组类人胶原蛋白/丝素蛋白支架并促进细胞与成骨细胞的相容性。


在此基础上，制备了一种新型的高孔隙率、孔径可控的重组类人胶原蛋白（RHLC）/丝素蛋白支架材料。体外测试了成骨细胞（OB）与混合物的相容性。通过扫描电镜和激光共聚焦显微镜观察支架细胞的形态、粘附和生长情况。培养 7 天后进行了广泛的测量，包括 3-[4, 5-二甲基噻唑-2-酰基]-2、5-二苯基四溴酸盐测定、细胞内总蛋白含量和碱性磷酸酶活性测定。 RHLC/丝心蛋白支架的存活率和蛋白质含量增加。 LSCM和SEM结果证实细胞在混合支架中比纯丝支架中生长得更好，并且表明细胞在RHLC/丝支架中更容易粘附和扩散。 RHLC/丝素蛋白支架是用于骨组织工程的有前景的生物材料。