Fluorophosphonate-Based Degrader Identifies Degradable Serine Hydrolases by Quantitative Proteomics.,ChemBioChem

当前位置： X-MOL 学术 › ChemBioChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fluorophosphonate-Based Degrader Identifies Degradable Serine Hydrolases by Quantitative Proteomics.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-06-05 , DOI: 10.1002/cbic.202000253
S Denise Field ₁ , Wankyu Lee ₁ , Jason K Dutra ₂ , Finley Scott F Serneo ₃ , Jon Oyer ₃ , Hua Xu ₁ , Douglas S Johnson ₁ , Christopher W Am Ende ₂ , Uthpala Seneviratne ₁

Affiliation

One and only one: The degradation susceptibility of serine hydrolases was assessed by using a probe that contained a fluorophosphonate warhead and a cereblon‐binding pomalidomide. A quantitative proteomics approach was employed to discover that ∼40 serine hydrolases were engaged but only a single serine hydrolase, lysophospholipase II, was degraded.

中文翻译：

基于氟膦酸盐的降解剂通过定量蛋白质组学鉴定可降解的丝氨酸水解酶。

一个，也是唯一一个：丝氨酸水解酶的降解敏感性是通过使用含有氟膦酸盐战斗部和结合脑神经的pomalidomide的探针进行评估的。采用定量蛋白质组学方法发现使用了约40种丝氨酸水解酶，但仅降解了一种丝氨酸水解酶溶血磷脂酶II。

更新日期：2020-06-05

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11