Ultrasonic-assisted ultrafiltration (UAU) removing bacterial endotoxin from diammonium glycyrrhizinate, was firstly applied to surfactant separation. Separation efficiency was related with four variables, including ultrafiltration molecular weight cut off (MWCO), ultrasonic power, concentration and pH. The SCQ-9200E ultrasonic system was provided for the study with adjustable ultrasonic power 80 W to 800 W, and the ultrasonic frequency was 40 KHz. On the basis of response surface methodology (RSM), the optimal separation conditions were determined to be the ultrafiltration MWCO as 10 kDa, the ultrasonic power as 570 W, diammonium glycyrrhizinate concentration as 150.00 μg/mL and the pH as 4.70. The experimental rejection of bacterial endotoxin was 94.08%, meanwhile the transmittance of diammonium glycyrrhizinate was 93.65%. Based on the ultrasonic power, solution volume, and ultrasonic container size, the experiments with UAU at different power intensities showed that ultrasonic at a power intensity of 57 W/L and the power density of 0.32 W/cm² could solve the separation contradiction between diammonium glycyrrhizinate and bacterial endotoxin. This study indicated that UAU could be an innovation in ultrasonic separation fields, and had a vast range of prospects for making use in pharmaceutical preparation area.

超声辅助超滤（UAU）从甘草酸二铵中去除细菌内毒素首先应用于表面活性剂的分离。分离效率与四个变量有关，包括超滤截留分子量（MWCO），超声功率，浓度和pH。为研究提供了SCQ-9200E超声系统，超声功率为80 W至800 W，超声频率为40 KHz。根据响应面法（RSM），确定最佳分离条件为超滤MWCO为10 kDa，超声功率为570 W，甘草酸二铵浓度为150.00μg/ mL，pH为4.70。实验中细菌内毒素的排斥率为94.08％，而甘草次酸二铵的透射率为93.65％。²可以解决甘草酸二铵与细菌内毒素的分离矛盾。这项研究表明，UAU可能是超声分离领域的一项创新，并在药物制备领域具有广阔的应用前景。