Short-term effects of the allelochemical umbelliferone on Triticum durum L. metabolism through GC-MS based untargeted metabolomics,Plant Science

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Short-term effects of the allelochemical umbelliferone on Triticum durum L. metabolism through GC-MS based untargeted metabolomics
Plant Science ( IF 4.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.plantsci.2020.110548
Biswapriya B Misra ₁ , Vivek Das ₂ , M Landi ₃ , M R Abenavoli ₄ , Fabrizio Araniti ₄

Affiliation

The present study used untargeted metabolomics to investigate the short-term metabolic changes induced in wheat seedlings by the specialized metabolite umbelliferone, an allelochemical. We used 10 day-old wheat seedlings treated with 104 μM umbelliferone over a time course experiment covering 6 time points (0 h, 6 h, 12 h, 24 h, 48 h, and 96 h), and compared the metabolomic changes to control (mock-treated) plants. Using gas chromatography mass spectrometry (GCMS)-based metabolomics, we obtained quantitative data on 177 metabolites that were derivatized (either derivatized singly or multiple times) or not, representing 139 non-redundant (unique) metabolites. Of these 139 metabolites, 118 were associated with a unique Human Metabolome Database (HMDB) identifier, while 113 were associated with a Kyoto Encyclopedia of Genes and Genomes (KEGG) identifier. Relative quantification of these metabolites across the time-course of umbelliferone treatment revealed 22 compounds (sugars, fatty acids, secondary metabolites, organic acids, and amino acids) that changed significantly (repeated measures ANOVA, P-value < 0.05) over time. Using multivariate partial least squares discriminant analysis (PLS-DA), we showed the grouping of samples based on time-course across the control and umbelliferone-treated plants, whereas the metabolite-metabolite Pearson correlations revealed tightly formed clusters of umbelliferone-derived metabolites, fatty acids, amino acids, and carbohydrates. Also, the time-course umbelliferone treatment revealed that phospho-l-serine, maltose, and dehydroquinic acid were the top three metabolites showing highest importance in discrimination among the time-points. Overall, the biochemical changes converge towards a mechanistic explanation of the plant metabolic responses induced by umbelliferone. In particular, the perturbation of metabolites involved in tryptophan metabolism, as well as the imbalance of the shikimate pathways, which are strictly interconnected, were significantly altered by the treatment, suggesting a possible mechanism of action of this natural compound.

中文翻译：

通过基于 GC-MS 的非靶向代谢组学研究化感化学伞形酮对硬粒小麦代谢的短期影响

本研究使用非靶向代谢组学来研究特殊代谢物伞形酮（一种化感物质）在小麦幼苗中引起的短期代谢变化。我们在涵盖 6 个时间点（0 小时、6 小时、12 小时、24 小时、48 小时和 96 小时）的时间过程实验中使用了用 104 μM 伞形酮处理的 10 天龄小麦幼苗，并将代谢组学变化与对照进行了比较（模拟处理的）植物。使用基于气相色谱质谱 (GCMS) 的代谢组学，我们获得了 177 种衍生（单独或多次衍生）代谢物的定量数据，代表 139 种非冗余（独特）代谢物。在这 139 种代谢物中，118 种与唯一的人类代谢组数据库 (HMDB) 标识符相关，而 113 个与京都基因和基因组百科全书 (KEGG) 标识符相关联。在伞形酮治疗的整个时间过程中对这些代谢物的相对量化显示 22 种化合物（糖、脂肪酸、次级代谢物、有机酸和氨基酸）随着时间的推移发生显着变化（重复测量方差分析，P 值 < 0.05）。使用多元偏最小二乘判别分析 (PLS-DA)，我们显示了基于对照和伞形酮处理植物的时间过程的样品分组，而代谢物-代谢物 Pearson 相关性显示紧密形成的伞形酮衍生代谢物簇，脂肪酸、氨基酸和碳水化合物。此外，时间过程伞形酮治疗表明磷酸-l-丝氨酸、麦芽糖、和脱氢奎尼酸是前三种代谢物，在时间点的区分中显示出最高的重要性。总体而言，生化变化趋向于对伞形酮诱导的植物代谢反应的机械解释。特别是，参与色氨酸代谢的代谢物的扰动，以及严格相互关联的莽草酸途径的不平衡，都因治疗而显着改变，表明这种天然化合物的可能作用机制。

更新日期：2020-09-01

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