The effects of recipient cell growth temperature, vector choice, and DNA methylation on transformation efficiency were explored for Lactiplantibacillus plantarum strain B38 and Apilactobacillus kunkeei strains YH15 and 3L. All three parameters significantly affected transformation efficiency. L. plantarum B38 and A. kunkeei YH15 transformed at higher efficiencies with the pTW8 vector than with the pTRKH2 vector; conversely, A. kunkeei 3L transformed at higher efficiency with pTRKH2. Mean transformation efficiencies as high as 7.8 × 10⁵ colony forming units (CFU) μg⁻¹ were obtained with pTW8 for B38, as high as 1.2 × 10⁵ CFU μg⁻¹ with pTW8 for YH15, and as high as 3.4 × 10⁶ CFU μg⁻¹ with pTRKH2 for 3L. With respect to methylation, B38 and YH15 transformed at higher efficiencies with DNA that lacked dam methylation, while 3L transformed at higher efficiency with DNA that was dam methylated. Methylation at Escherichia coli dcm sites did not affect the ability of pTRKH2 or pTW8 to transform these strains. Recipient cell growth at 21 °C rather than at 37 °C significantly increased transformation efficiencies when using each strain's preferred vector and methylation state; pTW8 without dam methylation for B38 and YH15 and pTRKH2 with dam methylation for 3L.

探讨了受体细胞生长温度，载体选择和DNA甲基化对植物乳杆菌B38菌株和Kapeei枯草芽孢杆菌YH15和3L转化效率的影响。所有这三个参数均显着影响转化效率。用pTW8载体比用pTRKH2载体以更高的效率转化了植物乳杆菌B38和A. kunkeei YH15。相反，pTRKH2使A. kunkeei 3L转化效率更高。平均转化效率高达7.8×10 ⁵菌落形成单位（CFU）微克^-1与pTW8为B38获得，高达1.2×10 ⁵  CFU微克^-1与pTW8为YH15，和高达3.4×10 ⁶  CFU微克^-1与pTRKH2为3L。关于甲基化，B38和YH15用缺少大坝甲基化的DNA转化效率更高，而3L用大坝甲基化的DNA转化效率更高。大肠杆菌dcm位点的甲基化不会影响pTRKH2或pTW8转化这些菌株的能力。当使用每种菌株的首选载体和甲基化状态时，受体细胞在21°C而不是37°C的生长显着提高了转化效率。对于B38和YH15，没有dam甲基化的pTW8和对于3L带有dam甲基化的pTRKH2 。