Neutrophils are an essential cellular component of the innate immune system, responsible for multiple effector mechanisms and aspects of inflammation. Neutrophil priming results in a rapid elevation in antimicrobial activities and can be measured by reactive oxygen species production, bacterial endocytosis, and de-novo synthesis of components such as interleukins. Mannose binding lectin (MBL), a C-type lectin pathogen recognition receptor is associated with immune functions including complement activation, opsonization and modulating immune responses. Whether MBL opsonization of pathogen can induce neutrophil priming has not been studied so far. Hence, studies were performed using MBL and neutrophils of Capra hircus (domestic goat) to evaluate the effects of MBL + MASPs interactions on neutrophil functions. It was found that MBL + MASPs opsonization of zymosan stimulates neutrophil functions including increased oxidative burst, enhanced endocytosis and modulates the expression level of NCF4, XBP1, CCL2, and CR1 genes. The results suggest that MBL-MASP complex can regulate neutrophil functioning.

中性粒细胞是先天免疫系统的重要细胞成分，负责多种效应机制和炎症方面。中性粒细胞引发导致抗菌活性快速升高，可以通过活性氧的产生、细菌内吞作用和白细胞介素等成分的从头合成来测量。甘露糖结合凝集素 (MBL) 是一种 C 型凝集素病原体识别受体，与免疫功能相关，包括补体激活、调理作用和调节免疫反应。到目前为止，尚未研究病原体的 MBL 调理作用是否可以诱导中性粒细胞启动。因此，使用 MBL 和Capra hircus 的中性粒细胞进行研究（家山羊）评估 MBL + MASP 相互作用对中性粒细胞功能的影响。发现酵母聚糖的 MBL + MASP 调理作用刺激中性粒细胞功能，包括增加氧化爆发、增强内吞作用并调节 NCF4、XBP1、CCL2 和 CR1 基因的表达水平。结果表明 MBL-MASP 复合物可以调节中性粒细胞功能。