Linalool is an important terpenoids of floral scents and has wide applications. In the past, several groups reported on a strategy to establish biosynthesis of linalool in yeast based on co-expression of Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 and Actinidia arguta linalool synthase LIS. However, ERG20 has both geranyl diphosphate synthase and farnesyl diphosphate synthase activities, which can lead to metabolic flow to farnesyl diphosphate. In this study, a heterologous linalool biosynthesis pathway was constructed in Escherichia coli and showed that using Abies grandis geranyl diphosphate synthase GPPS2 instead of ERG20 can effectively improve linalool biosynthesis. Subsequently, we further improved the biosynthesis of linalool by overexpression of isopentenyl diphosphate isomerase Idi.

中文翻译：

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从工程大肠杆菌中的葡萄糖从头生物合成芳樟醇

芳樟醇是一种重要的花香萜类化合物，用途广泛。过去，几个研究小组报道了基于酿酒酵母法呢基二磷酸合酶 ERG20 和 Actinidia arguta 芳樟醇合成酶 LIS 的共表达在酵母中建立芳樟醇生物合成的策略。然而，ERG20 同时具有香叶基二磷酸合酶和法呢基二磷酸合酶活性，这可导致代谢流向二磷酸法呢基。本研究在大肠杆菌中构建了异源芳樟醇生物合成途径，表明使用大冷杉香叶基二磷酸合酶GPPS2代替ERG20可以有效提高芳樟醇的生物合成。随后，我们通过异戊烯基二磷酸异构酶 Idi 的过表达进一步改进了芳樟醇的生物合成。