Abstract This study is about the use of the boron-doped diamond electrode pretreated electrochemically (anodic and subsequent cathodic) in the voltammetric determination of hepatitis C antiviral drug ledipasvir. Cyclic voltammetric measurements revealed that the compound yielded two well-separated irreversible oxidation peaks (PA1 and PA2) in acidic medium. The dependence of intensities of currents and potentials on electrode pretreatment, pH and nature of the supporting electrolyte, and other variables was investigated by square-wave voltammetry. Determination of ledipasvir was performed in Britton-Robinson buffer (pH 2.0) on the basis of both PA1 and PA2, which offered linear concentration ranges 0.5–60.0 μg mL−1 (5.6 × 10−7–6.8 × 10−5 mol L−1) and 5.0–60.0 μg mL−1 (5.6 × 10−6–6.8 × 10−5 mol L−1), respectively, with detection limits of 0.12 μg mL−1 (1.4 × 10−7 mol L−1) and 0.76 μg mL−1 (8.6 × 10−7 mol L−1). The practical applicability of developed methodology was tested by the assay of a fixed-dose combination tablet with sofosbuvir.

中文翻译：

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丙型肝炎抗病毒药物ledipasvir在非改性掺硼金刚石电极上的电分析研究和测定

摘要 本研究是关于使用经电化学（阳极和随后阴极）预处理的掺硼金刚石电极在丙型肝炎抗病毒药物雷迪帕韦伏安法测定中的应用。循环伏安测量表明，该化合物在酸性介质中产生了两个分离良好的不可逆氧化峰（PA1 和 PA2）。通过方波伏安法研究了电流和电位强度对电极预处理、支持电解质的 pH 值和性质以及其他变量的依赖性。在基于 PA1 和 PA2 的 Britton-Robinson 缓冲液 (pH 2.0) 中测定 ledipasvir，其线性浓度范围为 0.5-60.0 μg mL-1 (5.6 × 10-7-6.8 × 10-5 mol L- 1) 和 5.0–60.0 μg mL−1 (5.6 × 10−6–6.8 × 10−5 mol L−1)，检测限为 0。12 μg mL-1 (1.4 × 10-7 mol L-1) 和 0.76 μg mL-1 (8.6 × 10-7 mol L-1)。已开发方法的实际适用性通过对固定剂量复方片剂与索非布韦的测定进行了测试。