Using Ringer solution as extender, the present study examined the protective effect of dimethyl sulphoxide (Me2SO; 8-12%, v/v) on the cryopreservation of giant grouper (Epinephelus lanceolatus) sperm. The cryopreserved sperm was then successfully applied in interspecific hybridization with tiger grouper (E. fuscoguttatus). Higher motility (90.56 ± 6.58%) and fertilization rate (69.61 ± 4.83%) was achieved in 10% Me2SO with Ringer solution as extender (dilution ratio 1:1), which should no significant difference in comparison with fresh sperm (95.88 ± 1.64% and 73.10 ± 1.28%). There were no statistical differences in both fertilization and hatching rates between hybrid and non-hybrid tiger grouper by using cryopreserved sperm for fertilization, but malformation rate of the hybrid was higher than non-hybrid (17%) (P < 0.05). Survival rate of the hybrid was lower than that of the controls at 15 days post hatching (23% vs 48%). However, hybrids showed survival rate equal to the controls at the end of the 60-day study period. Hybridization of E. fuscoguttatus x E. lanceolatus was successfully achieved using cryopreserved sperm from giant grouper. The cryopreservation of giant grouper sperm and its application in hybridization provided a technical support for further grouper breeding work.

中文翻译：

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虎斑石斑鱼(Epinephelus fuscoguttatus ♀) x 巨石斑鱼(Epinephelus lanceolatus ♂) 的冷冻精子杂交

本研究使用林格溶液作为增量剂，研究了二甲基亚砜（Me2SO；8-12%，v/v）对巨型石斑鱼（Epinephelus lanceolatus）精子的冷冻保存的保护作用。然后将冷冻保存的精子成功应用于与虎斑石斑鱼（E. fuscoguttatus）的种间杂交。以林格溶液为稀释剂（稀释比 1:1）在 10% Me2SO 中实现了更高的运动性 (90.56 ± 6.58%) 和受精率 (69.61 ± 4.83%)，与新鲜精子 (95.88 ± 1.64) 相比应该没有显着差异% 和 73.10 ± 1.28%）。杂交虎斑石斑鱼与非杂交虎斑石斑鱼冻存精子受精受精率和孵化率均无统计学差异，但杂交虎斑鱼畸形率高于非杂交虎斑鱼（17%）（P < 0.05）。在孵化后 15 天，杂交种的存活率低于对照（23% 对 48%）。然而，在 60 天研究期结束时，杂种的存活率与对照组相同。E. fuscoguttatus x E. lanceolatus 的杂交使用来自巨型石斑鱼的冷冻保存的精子成功地实现。巨石斑鱼精子的冷冻保存及其在杂交中的应用为石斑鱼的进一步繁育工作提供了技术支持。