Lead, an environmental toxicant in our daily life, has been reported to have adverse effectson human and animals. Previous studies showed lead could accumulate in kidney tissues of carp and cause injury to the kidney tissues. In this study, we aimed to investigate the protective effects of andrographolide on lead-induced kidney injury in common carp and to clarify the possible mechanism. The results showed that treatment of andrographolide significantly inhibited lead-induced TNF-α and IL-1β production. Meanwhile, the MPO activity and MDA content were also inhibited by andrographolide. The activities of GSH and SOD were decreased and treatment of andrographolide significantly up-regulated GSH and SOD activities. Furthermore, qRT-PCR results showed andrographolide dose-dependently suppressed lead-induced NF-κB activation. Andrographolide increased Nrf2 and HO-1 expression in a dose-dependent manner. In conclusion, this study we found andrographolide could protect carp against lead-induced kidney injury through inhibiting inflammatory and oxidative responses.

据报道，铅是我们日常生活中的一种环境毒物，对人类和动物有不利影响。先前的研究表明，铅可能在鲤鱼的肾脏组织中积聚，并导致肾脏组织损伤。在这项研究中，我们旨在研究穿心莲内酯对铅引起的鲤鱼肾脏损伤的保护作用，并阐明可能的机制。结果表明，穿心莲内酯治疗显着抑制了铅诱导的TNF-α和IL-1β的产生。同时，穿心莲内酯还抑制了MPO活性和MDA含量。谷胱甘肽和超氧化物歧化酶的活性下降，穿心莲内酯的治疗显着上调了谷胱甘肽和超氧化物歧化酶的活性。此外，qRT-PCR结果显示穿心莲内酯剂量依赖性地抑制了铅诱导的NF-κB活化。穿心莲内酯以剂量依赖性方式增加Nrf2和HO-1表达。总之，这项研究发现穿心莲内酯可以通过抑制炎症和氧化反应来保护鲤鱼免受铅引起的肾脏损伤。