Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial cells (HUVECs). Subsequently, changes in the cytoskeletal structure and its binding proteins were analyzed by immunofluorescence and real-time PCR, respectively. The results showed that FB1 could inhibit the viability of HUVECs in a dose-dependent manner. After treatment of HUVECs with FB1, the hypotonic resistance, cell surface charges, cell membrane fluidity, and migration ability were weakened, whereas the ROS levels were significantly increased. Moreover, the cytoskeletal structure of the HUVECs was significantly changed, and the mRNA expression of some important actin-binding proteins was altered. Therefore, this study revealed that FB1 can affect the migration and cytoskeletal structure of HUVECs, which provides a new perspective for further understanding the molecular mechanisms of FB1 toxicity.

中文翻译：

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Fumonisin B1 影响人脐静脉内皮细胞的生物物理特性、迁移和细胞骨架结构。

伏马菌素B1（FB1）是自然界中一种重要的真菌毒素，严重威胁着人类和动物的健康，但其具体的毒性作用靶点和分子机制以及潜在的致癌性尚不清楚。在这项研究中，我们首先检测了 FB1 对人脐静脉内皮细胞 (HUVEC) 的细胞活力、生物物理特性、迁移能力和活性氧 (ROS) 的影响。随后，分别通过免疫荧光和实时 PCR 分析了细胞骨架结构及其结合蛋白的变化。结果表明，FB1能以剂量依赖的方式抑制HUVECs的活力。FB1处理HUVECs后，低渗阻力、细胞表面电荷、细胞膜流动性和迁移能力减弱，而ROS水平显着升高。此外，HUVEC 的细胞骨架结构发生了显着变化，一些重要的肌动蛋白结合蛋白的 mRNA 表达也发生了改变。因此，本研究揭示了FB1可以影响HUVECs的迁移和细胞骨架结构，为进一步了解FB1毒性的分子机制提供了新的视角。