Key message

We identified 15 genes encoding enzymes for phenylpropanoid biosynthesis in the genome of birch by combining phylogenetic analysis and tissue-specific expression data.

Abstract

Lignin is one of the most abundant terrestrial biopolymers and is essential for plant structure and defense. An essential step in lignin formation is phenylpropanoid synthesis, we identified 120 gene models in 10 protein families encoding enzymes for phenylpropanoid biosynthesis in the genome of Betula pendula. The transcript abundance was determined for all 120 genes in xylem, root, leaf, and flower tissues using RNA-seq technology. We identified 15 genes that likely encode phenylpropanoid biosynthesis enzymes during wood formation. Ten of these genes are evolutionarily conserved compared to the lignin genes in Populus trichocarpa.

中文翻译：

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西南桦中推定的木质素生物合成基因的鉴定

关键信息

通过结合系统发育分析和组织特异性表达数据，我们在桦树基因组中鉴定了15种编码苯丙烷类生物合成酶的基因。

抽象

木质素是最丰富的陆地生物聚合物之一，对植物结构和防御至关重要。木质素形成的重要步骤是苯丙烷的合成，我们在10个蛋白质家族中鉴定了120个基因模型，这些家族编码了桦木基因组中苯丙烷生物合成酶。使用RNA-seq技术确定了木质部，根，叶和花组织中所有120个基因的转录本丰度。我们确定了15个可能在木材形成过程中编码苯丙烷生物合成酶的基因。与毛果杨中的木质素基因相比，这些基因中有10个在进化上是保守的。