Betalain is a natural pigment with serious antioxidant and biological activities currently used as a food colourant, however; the isolation of this compound is difficult because of its instability. The research was aimed to synthesise and characterise the copolymeric adsorbents for the recovery of betalains by means of the solid-phase extraction (SPE) technique. For this purpose, divinylbenzene (crosslinker) was polymerised with polyvinylpyrrolidone containing vinyl groups by using free radical polymerisation, and toluene was used as a porogene. Polymeric resins in different polyvinylpyrrolidone/divinylbenzene (PVP/DVB) and toluene/divinylbenzene (Tol/DVB) rates were synthesised. The effects of these parameters on the polymerisation efficiency, size distribution, morphology, swelling properties and adsorption capacities of adsorbents were investigated in the later characterization stage. Adsorption tests were conducted by analyzing phenol adsorption in batch model systems. The KOD-4 coded adsorbent exhibited the best performance in adsorption tests with PVP/DVB and Tol/DVB ratios of 0.5 and 1.5, respectively. The betalains were recovered strongly from the red beet juice via the packed bed adsorption column system filled with KOD-4. Then, column reusability and regeneration were studied. In total, the prepared column system was used five times. Ultimately, the results of this study can lead to wide-scale application of betalain-enriched beetroot extracts in the food, pharmaceutical, and cosmetic industries due to their antioxidant and colourant properties as well as their biological activities.

Betalain是一种天然色素，具有严重的抗氧化剂和生物活性，目前已用作食用色素。由于其不稳定，很难分离出该化合物。这项研究旨在通过固相萃取（SPE）技术合成和表征用于回收甜菜碱的共聚吸附剂。为此，通过自由基聚合，使二乙烯基苯（交联剂）与含有乙烯基的聚乙烯吡咯烷酮聚合，并使用甲苯作为光气。合成了不同聚乙烯吡咯烷酮/二乙烯基苯（PVP / DVB）和甲苯/二乙烯基苯（Tol / DVB）速率的聚合树脂。这些参数对聚合效率，尺寸分布，形态，在表征后期，研究了吸附剂的溶胀性能和吸附能力。通过在批处理模型系统中分析苯酚吸附来进行吸附测试。KOD-4编码的吸附剂在吸附测试中表现出最好的性能，PVP / DVB和Tol / DVB的比率分别为0.5和1.5。通过填充了KOD-4的填充床吸附柱系统从甜菜汁中强烈回收了甜菜碱。然后，对色谱柱的可重用性和再生进行了研究。所制备的色谱柱系统总共使用了五次。最终，这项研究的结果由于其抗氧化剂和着色剂的特性以及它们的生物活性，可以使富含甜菜碱的甜菜根提取物在食品，制药和化妆品行业得到广泛应用。