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Impact of interleukin IL-6 rs-1474347 and IL-10 rs-1800896 genetic polymorphisms on the susceptibility of HCV-infected Egyptian patients to hepatocellular carcinoma.
Immunologic Research ( IF 3.3 ) Pub Date : 2020-06-04 , DOI: 10.1007/s12026-020-09126-8
Reham M Abd El-Baky 1 , Helal F Hetta 2, 3 , Gopala Koneru 3 , Marwa Ammar 4 , Engy A Shafik 5 , Dina A Mohareb 6 , Muhammad Abbas El-Masry 7 , Haidi K Ramadan 8 , Mohamed Zakaria Abu Rahma 8 , Michael A Fawzy 9 , Moustafa Fathy 9, 10
Affiliation  

Hepatitis C virus (HCV) is considered leading cause of cirrhosis and hepatocellular carcinoma (HCC). We aimed to examine the association of IL-6 and IL-10 single-nucleotide polymorphisms with the progression of chronic HCV (CHC) infection to cirrhosis and HCC. For comparative purposes, four groups were enrolled; chronic HCV group (CHC, n = 22), HCV-related liver cirrhosis group (HCV-LC, n = 22), HCV-related HCC group (HCV-HCC, n = 54), and an apparently healthy control group (controls, n = 48). HCC diagnosis and staging were in concordance to Barcelona Clinic Liver Cancer (BCLC) staging system. IL-6 rs-1474347 and IL-10 rs-1800896 genotyping was performed by allelic (VIC- and FAM-labeled) discrimination method using assay-on-demand TaqMan real-time PCR assays. For IL-6 rs1474347, the AA genotype was more frequent in CHC, HCV-LC, and HCV-HCC compared to controls. Also, the IL-6 rs1474347 AC genotype was favorable for the progression of HCV chronic infection to cirrhosis and HCC. On the other hand, the IL-10 rs1800896 TT genotype was found to be prominent in the HCC group. Additionally, the IL-10 rs180096 TT genotype was favorable for the progression of chronic HCV infection to cirrhosis and HCC. Furthermore, higher levels of AFP were observed in HCC patients with IL-6 rs1474347 AA genotype and HCC patients with IL-10 rs1800896 CC and TT genotypes. Screening for IL-6 rs 1474347 AC genotype and IL-10 rs180096 TT genotype as well as the determination of AFP level showed to be good markers for examining the susceptibility of HCV Egyptian patients to develop cirrhosis and HCC.



中文翻译:

白细胞介素 IL-6 rs-1474347 和 IL-10 rs-1800896 基因多态性对 HCV 感染的埃及患者对肝细胞癌易感性的影响。

丙型肝炎病毒 (HCV) 被认为是导致肝硬化和肝细胞癌 (HCC) 的主要原因。我们旨在检查 IL-6 和 IL-10 单核苷酸多态性与慢性 HCV (CHC) 感染进展为肝硬化和 HCC 的关联。出于比较的目的,招募了四组;慢性 HCV 组(CHC,n  = 22)、HCV 相关肝硬化组(HCV-LC,n  = 22)、HCV 相关 HCC 组(HCV-HCC,n  = 54)和一个明显健康的对照组(对照组) , n = 48)。HCC 诊断和分期与巴塞罗那临床肝癌 (BCLC) 分期系统一致。IL-6 rs-1474347 和 IL-10 rs-1800896 基因分型通过等位基因(VIC 和 FAM 标记)鉴别方法使用按需测定 TaqMan 实时 PCR 测定进行。对于 IL-6 rs1474347,与对照相比,AA 基因型在 CHC、HCV-LC 和 HCV-HCC 中更为常见。此外,IL-6 rs1474347 AC 基因型有利于 HCV 慢性感染进展为肝硬化和 HCC。另一方面,发现 IL-10 rs1800896 TT 基因型在 HCC 组中很突出。此外,IL-10 rs180096 TT 基因型有利于慢性 HCV 感染进展为肝硬化和 HCC。此外,在具有 IL-6 rs1474347 AA 基因型的 HCC 患者和具有 IL-10 rs1800896 CC 和 TT 基因型的 HCC 患者中观察到更高水平的 AFP。筛选 IL-6 rs 1474347 AC 基因型和 IL-10 rs180096 TT 基因型以及测定 AFP 水平表明是检查 HCV 埃及患者发展为肝硬化和 HCC 的易感性的良好标志物。

更新日期:2020-06-04
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