Hepatitis C virus (HCV) is considered leading cause of cirrhosis and hepatocellular carcinoma (HCC). We aimed to examine the association of IL-6 and IL-10 single-nucleotide polymorphisms with the progression of chronic HCV (CHC) infection to cirrhosis and HCC. For comparative purposes, four groups were enrolled; chronic HCV group (CHC, n = 22), HCV-related liver cirrhosis group (HCV-LC, n = 22), HCV-related HCC group (HCV-HCC, n = 54), and an apparently healthy control group (controls, n = 48). HCC diagnosis and staging were in concordance to Barcelona Clinic Liver Cancer (BCLC) staging system. IL-6 rs-1474347 and IL-10 rs-1800896 genotyping was performed by allelic (VIC- and FAM-labeled) discrimination method using assay-on-demand TaqMan real-time PCR assays. For IL-6 rs1474347, the AA genotype was more frequent in CHC, HCV-LC, and HCV-HCC compared to controls. Also, the IL-6 rs1474347 AC genotype was favorable for the progression of HCV chronic infection to cirrhosis and HCC. On the other hand, the IL-10 rs1800896 TT genotype was found to be prominent in the HCC group. Additionally, the IL-10 rs180096 TT genotype was favorable for the progression of chronic HCV infection to cirrhosis and HCC. Furthermore, higher levels of AFP were observed in HCC patients with IL-6 rs1474347 AA genotype and HCC patients with IL-10 rs1800896 CC and TT genotypes. Screening for IL-6 rs 1474347 AC genotype and IL-10 rs180096 TT genotype as well as the determination of AFP level showed to be good markers for examining the susceptibility of HCV Egyptian patients to develop cirrhosis and HCC.

丙型肝炎病毒 (HCV) 被认为是导致肝硬化和肝细胞癌 (HCC) 的主要原因。我们旨在检查 IL-6 和 IL-10 单核苷酸多态性与慢性 HCV (CHC) 感染进展为肝硬化和 HCC 的关联。出于比较的目的，招募了四组；慢性 HCV 组（CHC，n  = 22）、HCV 相关肝硬化组（HCV-LC，n  = 22）、HCV 相关 HCC 组（HCV-HCC，n  = 54）和一个明显健康的对照组（对照组） , n = 48）。HCC 诊断和分期与巴塞罗那临床肝癌 (BCLC) 分期系统一致。IL-6 rs-1474347 和 IL-10 rs-1800896 基因分型通过等位基因（VIC 和 FAM 标记）鉴别方法使用按需测定 TaqMan 实时 PCR 测定进行。对于 IL-6 rs1474347，与对照相比，AA 基因型在 CHC、HCV-LC 和 HCV-HCC 中更为常见。此外，IL-6 rs1474347 AC 基因型有利于 HCV 慢性感染进展为肝硬化和 HCC。另一方面，发现 IL-10 rs1800896 TT 基因型在 HCC 组中很突出。此外，IL-10 rs180096 TT 基因型有利于慢性 HCV 感染进展为肝硬化和 HCC。此外，在具有 IL-6 rs1474347 AA 基因型的 HCC 患者和具有 IL-10 rs1800896 CC 和 TT 基因型的 HCC 患者中观察到更高水平的 AFP。筛选 IL-6 rs 1474347 AC 基因型和 IL-10 rs180096 TT 基因型以及测定 AFP 水平表明是检查 HCV 埃及患者发展为肝硬化和 HCC 的易感性的良好标志物。