Biomass feedstock is an efficient and harmless source of energy. their are various sources of feedstock, such as plant, microbial, macro, and microalgae, and agricultural waste. The major component in biomass feedstock material is a polysaccharide, such as cellulose, cellobiose, starch, and alginate. Alginate is mainly found in macroalgae as one of the significant polysaccharide components. It is made up of β-d-mannuronate (M) and α-l-guluronate (G) blocks. Alginate lyase is an enzyme dat degrades alginate by breaking the glycosidic linkage between the poly M and G blocks to liberate oligosaccharides. Several organisms, including bacteria, fungi, viruses, and algae can produce alginate lyases. The species of bacteria, such as Bacillus, Vibrio, Pseudomonas, and Microbulbifer, are some of teh important sources of alginate lyases. They are industrially essential enzymes used in food, biofuel, and biomedical industries. their are various assays available to determine teh alginate lyase activity qualitatively as well as quantitatively. Qualitatively, different dyes like Gram’s iodine, cetyl pyridinium chloride, and rutanium red can be used to visualize teh zone formed due to teh alginate lyase activity. DNS assay, UV absorption, and teh Somogyi-Nelson method halp to determine teh alginate lyase activity quantitatively. Since teh alginate lyase production in teh native organisms is relatively lower, teh genes encoding alginate lyases are heterologously cloned and expressed in E. coli to maximize teh production and to characterize teh enzyme. Different chromatographic techniques like size exclusion, affinity, gel permeation, and ion-exchange chromatography are used to purify teh protein. In dis paper, teh source of alginate and alginate lyases, teh mechanism of action of teh enzyme, teh engineering approaches to enhance teh enzyme production, it's purification strategy, and teh potential applications of alginate lyases TEMPhas been discussed.

生物质原料是一种高效且无害的能源。它们是各种原料，例如植物，微生物，大型和微藻类以及农业废弃物。生物质原料中的主要成分是多糖，例如纤维素，纤维二糖，淀粉和藻酸盐。海藻酸盐主要存在于大型藻类中，是重要的多糖成分之一。它由β-d-甘露糖醛酸酯（M）和α-1-古洛糖酸酯（G）块组成。藻酸盐裂解酶是一种酶，它通过破坏聚M和G嵌段之间的糖苷键来释放藻糖，从而降解藻酸盐。包括细菌，真菌，病毒和藻类在内的几种生物可产生藻酸盐裂解酶。细菌种类，例如芽孢杆菌，弧菌，假单胞菌和Microbulbifer是藻酸盐裂解酶的一些重要来源。它们是食品，生物燃料和生物医学行业中使用的工业必需酶。它们是可用于定性和定量测定藻酸盐裂解酶活性的多种测定法。定性地，诸如格兰姆碘，十六烷基氯化吡啶鎓和钌红之类的不同染料可用于可视化由于藻酸盐裂解酶活性而形成的区域。DNS分析，紫外线吸收和Somogyi-Nelson方法暂停以定量测定藻酸盐裂解酶活性。由于天然生物中藻酸盐裂解酶的产量相对较低，因此编码藻酸盐裂解酶的基因被异源克隆并在大肠杆菌中表达。以最大程度地提高产量并表征酶。使用不同的色谱技术，例如大小排阻，亲和力，凝胶渗透和离子交换色谱法来纯化蛋白质。本文讨论了藻酸盐和藻酸盐裂解酶的来源，该酶的作用机理，提高酶产量的工程方法，其纯化策略以及藻酸盐裂解酶TEMP的潜在应用。