Abstract

Festuca species have been used for forage, turf, soil conservation and ornamental purpose, which highlighted the importance of these species. This study was conducted to determine genetic variability among the 48 accessions of fine-leaved fescue from different regions of Iran using ISSR markers. To understand genetic relationships among these accessions, unweight pair-group method (UPGMA) were applied to the ISSR marker data set. The ten ISSR primers amplified in total 189 loci for 48 Festuca ovina accessions. All of the amplified loci were polymorphic. A high level of genetic diversity was detected in F. ovina accessions with an average genetic diversity index of 1.522. The expected heterozygosity (He) for the primers varied from 0.153 to 0.347. According to the ISSR data, accessions grouped in two main and seven sub clusters. Results indicated that each of the main clusters include plants that demonstrate Green and Silver phenotypes. Little congruence between the groupings patterns of accessions of F. ovina based on ISSR markers data and categorization based on phenotypes as a Green and Silver, was observed. In conclusion results reveal the high genetic diversity among F. ovina accessions, which can be used for alternative conservation and breeding programs.

中文翻译：

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基于ISSR的优良羊茅（Festuca ovina L。）研究突出了伊朗种质的遗传多样性。

摘要

Festuca种已用于草料，草皮，水土保持和观赏目的，突出了这些种的重要性。进行这项研究以确定使用ISSR标记从伊朗不同地区的48片细叶羊茅中的遗传变异性。为了了解这些种质之间的遗传关系，对ISSR标记数据集应用了不重对组法（UPGMA）。十个ISSR引物在总共189个基因座中扩增了48个Festuca卵子。所有扩增的基因座都是多态的。在卵巢小球藻中检测到高水平的遗传多样性种的平均遗传多样性指数为1.522。引物的预期杂合度（He）在0.153至0.347之间。根据ISSR数据，种质分为两个主要和七个子类。结果表明，每个主要簇包括表现出绿色和银色表型的植物。基于ISSR标记数据的卵形葡萄球菌的分组模式与基于绿色和银色的表型分类之间几乎没有一致性。总之，结果揭示了卵形镰刀菌种之间的高度遗传多样性，可用于替代性保护和育种计划。