Abstract

Identification and molecular analysis of high molecular weight glutenin subunits (HMW-GS) of wild emmer wheat T. dicoccoides K5199 were carried out. Novel HMW glutenin subunits 1AxTd and 1ByTd were identified. Nucleotide sequences of 1AxTd and 1ByTd genes were detected (GenBank accession numbers MH475136 and MG897125, respectively). The coding sequence of 1AxTd was similar to 1Ax1 of T. aestivum (99.7%), while the 1ByTd gene sequence was similar to the genes of other T. dicoccoides samples (98.5 and 97.6%). Primary and secondary protein structures of the studied HMW-GS allowed for predicting a high contribution of the 1AxTd subunit to the breadmaking qualities and the average contribution of 1ByTd thereto. Evaluation of the most important grain quality traits identified increased protein and gluten content as well as reduced rheological properties of T. dicoccoides K5199 gluten.

中文翻译：

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野生Emmer小麦（Triticum dicoccoides）高分子量谷蛋白亚基的鉴定。

摘要

进行了野生Emmer小麦T. dicoccoides K5199高分子量谷蛋白亚基（HMW-GS）的鉴定和分子分析。确定了新型HMW谷蛋白亚基1AxTd和1ByTd。检测到1AxTd和1ByTd基因的核苷酸序列（GenBank登录号分别为MH475136和MG897125）。的编码序列1AxTd类似于1Ax1的普通小麦（99.7％），而1ByTd基因序列是相似的其它的基因T.野生二粒样本（98.5和97.6％）。研究的HMW-GS的一级和二级蛋白质结构可预测1AxTd亚基对面包制作质量的高贡献以及1ByTd对其的平均贡献。对最重要的谷物品质性状进行评估后，发现T. dicoccoides K5199面筋的蛋白质和面筋含量增加，流变性降低。