PRK1 is a member of the protein kinase C-related kinase (PRK) family of serine/threonine kinases and a downstream effector of Rho GTPases. PRK1 has three N-terminal Homology Region 1 (HR1) domains (HR1a, HR1b and HR1c), which form antiparallel coiled coils that interact with Rho family GTPases. PRK1 also has a C2-like domain that targets it to the plasma membrane and a kinase domain, which is a member of the protein kinase C superfamily. PRK1 is involved in cytoskeletal regulation, cell adhesion, cell cycle progression and the immune response, and is implicated in cancer. There is currently no structural information for the HR1c domain. The ¹H, ¹⁵N and ¹³C NMR backbone and sidechain resonance assignment of the HR1c domain presented here forms the basis for this domain’s structural characterisation. This work will also enable studies of interactions between the three HR1 domains in an effort to obtain structural insight into the regulation of PRK1 activity.

中文翻译：

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PRK1（一种蛋白激酶 C 相关激酶）的 HR1c 结构域的 1H、15N 和 13C 共振分配。

PRK1 是丝氨酸/苏氨酸激酶的蛋白激酶 C 相关激酶 (PRK) 家族的成员，也是 Rho GTPases 的下游效应子。PRK1 具有三个 N 端同源区域 1 (HR1) 域（HR1a、HR1b 和 HR1c），它们形成与 Rho 家族 GTPase 相互作用的反平行卷曲螺旋。PRK1 还具有一个 C2 样结构域，将其靶向质膜和一个激酶结构域，它是蛋白激酶 C 超家族的成员。PRK1 参与细胞骨架调节、细胞粘附、细胞周期进程和免疫反应，并与癌症有关。目前没有 HR1c 域的结构信息。的^1个H，¹⁵ N和¹³此处介绍的 HR1c 结构域的 C NMR 主链和侧链共振分配构成了该结构域结构表征的基础。这项工作还将使研究三个 HR1 域之间的相互作用成为可能，从而获得对 PRK1 活性调节的结构洞察。