As important plant growth-promoting rhizobacteria, phosphate-solubilizing bacteria (PSB) fix nitrogen, dissolve potassium, promote growth, improve the soil micro-environment, and enhance soil fertility. A high-efficiency PSB strain from the pine tree rhizosphere, Burkholderia multivorans WS-FJ9, was screened in our laboratory. In this study, using a Bio Screener fully automatic microbial growth curve meter to determine the growth of the WS-FJ9 strain in phosphate-removing medium, the growth and mineral phosphate solubilization of WS-FJ9 were measured by Mo-Sb colorimetry and organophosphate-solubilization plate assays. Second-generation sequencing technology was used to obtain genomic information and to analyze possible phosphate decomposition genes. The related expression levels of these genes under different soluble phosphate levels were determined by quantitative real-time PCR. The results showed that WS-FJ9 had strong adaptability and capacity for mineral phosphate solubilization at low soluble phosphate levels, which is characterized by its low soluble phosphate induction and high soluble phosphate inhibition. The amount of solubilized mineral phosphate could exceed 140 mg/L. The total length of the WS-FJ9 genome was 7,497,552 bp after splicing, and the GC content was 67.37%. Eight phosphate-related genes were selected to determine their expression patterns at different soluble phosphate levels. Among them, AP-2, GspE and GspF were only related to organic phosphate, HlyB was only related to inorganic phosphate, and PhoR, PhoA, AP-1 and AP-3 were related to both. The WS-FJ9 strain utilizes multiple pathways for mineral phosphate solubilization, and the solubilization processes of different phosphate sources are interrelated and independent, indicating that the WS-FJ9 strain can adapt to different phosphate source environments and has good potential for future applications.

作为重要的植物生长根瘤菌，磷酸盐增溶菌（PSB）可以固氮，溶解钾，促进生长，改善土壤微环境并增强土壤肥力。来自松树根际，伯克霍尔德氏菌的高效PSB菌株WS-FJ9在我们的实验室中进行了筛选。在这项研究中，使用Bio Screener全自动微生物生长曲线仪来确定WS-FJ9菌株在除磷培养基中的生长，并通过Mo-Sb比色法和有机磷酸酯法测定WS-FJ9的生长和矿物磷酸盐的溶解度。溶解板测定。第二代测序技术用于获得基因组信息并分析可能的磷酸盐分解基因。通过定量实时PCR确定在不同可溶性磷酸盐水平下这些基因的相关表达水平。结果表明，WS-FJ9在低可溶性磷酸盐水平下具有很强的适应性和对无机磷酸盐溶解的能力，其特征在于其低可溶性磷酸盐诱导和高可溶性磷酸盐抑制。溶解的矿物磷酸盐的量可能超过140 mg / L。剪接后，WS-FJ9基因组的全长为7497552 bp，GC含量为67.37％。选择了八个与磷酸盐有关的基因，以确定它们在不同可溶性磷酸盐水平下的表达模式。其中，AP - 2，GspE和GspF仅与有机磷酸盐有关，HlyB仅与无机磷酸盐有关，而PhoR，PhoA，AP - 1和AP - 3两者都有关。WS-FJ9菌株利用多种途径进行矿物磷酸盐的增溶，并且不同磷酸盐来源的增溶过程是相互关联和独立的，这表明WS-FJ9菌株可适应不同的磷酸盐来源环境，并具有未来应用的良好潜力。