A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine. At present, these files are mainly built from searching the literature and/or querying data sources focused on posttranslational modifications. Most glycoproteomics search engines include a default composition file that is readily used when processing mass spectrometry data. We introduce here a glycan composition visualising and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor. It has web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions. The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarising information searched in the database. These results provide a guide for selecting or deselecting compositions in a file in order to reflect the context of a study as closely as possible. As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application. It is also directly accessible from the GlyConnect database.

中文翻译：

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使用GlyConnect Compozitor检查和微调聚糖成分的选择

实现准确完整糖肽鉴定的关键是糖蛋白组学搜索引擎定义的聚糖成分文件，该文件可用于将实验质量与理论质量进行匹配。目前，这些文件主要是通过搜索文献和/或查询着眼于翻译后修饰的数据源来构建的。大多数糖蛋白组学搜索引擎都包括默认的成分文件，该文件在处理质谱数据时很容易使用。我们在这里介绍与GlyConnect数据库相关联的，称为GlyConnect Compozitor的聚糖成分可视化和比较工具。它具有Web界面，可通过该界面查询数据库以获取与一组聚糖成分有关的上下文信息。该工具利用通过共享单糖计数相互关联的成分的优势，并输出汇总了在数据库中搜索到的信息的交互式图表。这些结果为选择或取消选择文件中的成分提供了指南，以便尽可能接近地反映研究的内容。作为Glycomics @ ExPASy计划的工具集的一部分，Compozitor托管在https://glyconnect.expasy.org/compozitor/上，可以将其作为Web应用程序运行。也可以从GlyConnect数据库直接访问它。作为Glycomics @ ExPASy计划的工具集的一部分，Compozitor托管在https://glyconnect.expasy.org/compozitor/上，可以将其作为Web应用程序运行。也可以从GlyConnect数据库直接访问它。作为Glycomics @ ExPASy计划的工具集的一部分，Compozitor托管在https://glyconnect.expasy.org/compozitor/上，可以将其作为Web应用程序运行。也可以从GlyConnect数据库直接访问它。