Pattern Engineering of Living Bacterial Colonies Using Meniscus-Driven Fluidic Channels.,ACS Synthetic Biology

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Pattern Engineering of Living Bacterial Colonies Using Meniscus-Driven Fluidic Channels.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-06-03 , DOI: 10.1021/acssynbio.0c00146
Vasily Kantsler _{1,

2} , Elena Ontañón-McDonald ₃ , Cansu Kuey ₃ , Manjari J Ghanshyam ₃ , Maria Chiara Roffin ₁ , Munehiro Asally _{3,

4}

Affiliation

Creating adaptive, sustainable, and dynamic biomaterials is a forthcoming mission of synthetic biology. Engineering spatially organized bacterial communities has a potential to develop such bio-metamaterials. However, generating living patterns with precision, robustness, and a low technical barrier remains as a challenge. Here we present an easily implementable technique for patterning live bacterial populations using a controlled meniscus-driven fluidics system, named as MeniFluidics. We demonstrate multiscale patterning of biofilm colonies and swarms with submillimeter resolution. Utilizing the faster bacterial spreading in liquid channels, MeniFluidics allows controlled bacterial colonies both in space and time to organize fluorescently labeled Bacillus subtilis strains into a converged pattern and to form dynamic vortex patterns in confined bacterial swarms. The robustness, accuracy, and low technical barrier of MeniFluidics offer a tool for advancing and inventing new living materials that can be combined with genetically engineered systems, and adding to fundamental research into ecological, evolutional, and physical interactions between microbes.

中文翻译：

使用弯液面驱动的流体通道对活菌落进行模式工程。

创造适应性、可持续和动态的生物材料是合成生物学即将到来的使命。工程空间组织的细菌群落具有开发这种生物超材料的潜力。然而，以精确、稳健和低技术壁垒生成生活模式仍然是一个挑战。在这里，我们提出了一种易于实施的技术，用于使用受控弯液面驱动的流体系统（称为 MeniFluidics）对活细菌种群进行模式化。我们展示了具有亚毫米分辨率的生物膜菌落和群体的多尺度模式。利用液体通道中更快的细菌扩散，MeniFluidics 允许在空间和时间上受控的细菌菌落组织荧光标记的枯草芽孢杆菌菌株成收敛模式，并在密闭的细菌群中形成动态涡流模式。MeniFluidics 的稳健性、准确性和低技术壁垒为推进和发明可与基因工程系统相结合的新生命材料提供了一种工具，并增加了对微生物之间生态、进化和物理相互作用的基础研究。

更新日期：2020-06-19

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