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Genetic characterisation and population structure analysis of indigenous and exotic eggplant (Solanum spp) accessions using microsatellite markers
The Journal of Horticultural Science and Biotechnology ( IF 1.7 ) Pub Date : 2020-06-04 , DOI: 10.1080/14620316.2020.1763211
Laxman L. Nandi 1 , Partha Saha 1 , T.K. Behera 1 , Y.A. Lyngdoh 1 , A.D. Munshi 1 , N.D. Saha 2 , Firoz Hossain 3 , Arpan Bhowmik 4 , R.S. Pan 5 , Aakriti Verma 3 , B.S. Tomar 1
Affiliation  

ABSTRACT In this study, a total of 20 simple sequence repeat (SSR) markers were used to study the genetic diversity and population structure among 60 accessions of eggplant. Out of 20 SSR markers, 15 were found to be polymorphic which were subjected to statistical analysis by Power Marker and NTSYSPc software. The polymorphic SSR markers generated 46 alleles with an average of 3.06 alleles per locus. The PIC value varied from 0.12 to 0.47. The marker emf21N03 was most informative with PIC value of 0.57. The UPGMA-based dendrogram classified all the accessions into two major clusters comprising cultivated accessions and wild progenitor in cluster I and other wild accessions in cluster II. The PCA plot separated the accessions into four quadrangles where unexploited wild accessions presented in second quadrant. Population structure analysis showed 6 sub- populations with 16 accessions in population I, 12 accessions in population II, 11 accessions in population III, 5 accessions in population IV, 12 accessions in population V and 4 in population VI. The subpopulation IV had all pure individuals. These findings will provide new insights in genetic resources conservation and exploitation of genetic diversity of eggplant germplasm in breeding programme.

中文翻译:

使用微卫星标记的本地和外来茄子(Solanum spp)种质的遗传特征和种群结构分析

摘要 本研究共利用20个简单序列重复(SSR)标记对60份茄子种质的遗传多样性和种群结构进行研究。在20个SSR标记中,发现15个是多态的,通过Power Marker和NTSYSPc软件进行统计分析。多态性 SSR 标记产生 46 个等位基因,每个基因座平均有 3.06 个等位基因。PIC 值从 0.12 到 0.47 不等。标记 emf21N03 的信息量最大,PIC 值为 0.57。基于 UPGMA 的树状图将所有种质分为两个主要集群,包括集群 I 中的栽培种质和野生祖先以及集群 II 中的其他野生种质。PCA 图将种质分成四个四边形,其中未开发的野生种质出现在第二象限中。种群结构分析显示6个亚种群,种群I 16份,种群II 12份,种群III 11份,种群IV 5份,种群V 12份,种群VI 4份。亚群 IV 全部为纯个体。这些发现将为遗传资源保护和育种计划中茄子种质遗传多样性的开发提供新的见解。
更新日期:2020-06-04
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