Grapevine trunk diseases (GTDs), which are associated with complex of xylem-inhabiting fungi, represent one of the major threats to vineyard sustainability currently. Botryosphaeria dieback, one of the major GTDs, is associated with wood colonization by Botryosphaeriaceae fungi, especially Neofusicoccum parvum. We used GC-MS and HPLC-MS to compare the wood metabolomic responses of the susceptible Vitis vinifera subsp. vinifera (V.v. subsp. vinifera) and the tolerant Vitis vinifera subsp. sylvestris (V.v. subsp. sylvestris) after artificial inoculation with Neofusicoccum parvum (N. parvum). N. parvum inoculation triggered major changes in both primary and specialized metabolites in the wood. In both subspecies, infection resulted in a strong decrease in sugars (fructose, glucose, sucrose), whereas sugar alcohol content (mannitol and arabitol) was enhanced. Concerning amino acids, N. parvum early infection triggered a decrease in aspartic acid, serine, and asparagine, and a strong increase in alanine and -alanine. A trend for more intense primary metabolism alteration was observed in V.v. subsp. sylvestris compared to V. v. subsp. vinifera. N. parvum infection also triggered major changes in stilbene and flavonoid compounds. The content in resveratrol and several resveratrol oligomers increased in the wood of both subspecies after infection. Interestingly, we found a higher induction of resveratrol oligomer (putative E-miyabenol C, vitisin C, hopeaphenol, ampelopsin C) contents after wood inoculation in V.v. subsp. sylvestris.

中文翻译：

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野生和栽培葡萄对树干新病原体小镰刀菌感染的木材代谢组学响应。

葡萄树干疾病（GTDs）与居住木质部的真菌复合体相关，是目前对葡萄园可持续性的主要威胁之一。Botryosphaeria dieback是主要的GTD之一，它与Botryosphaeriaceae真菌，尤其是小新镰刀菌（Neofusicoccum parvum）的木材定殖有关。我们使用GC-MS和HPLC-MS比较了易感的葡萄亚种的木材代谢组学响应。葡萄（VV亚种。葡萄）和宽容的葡萄亚种。樟子松（VV亚种。樟子松）后人工接种Neofusicoccum孢子虫（N.孢子虫）。小猪猪笼草的接种引发了木材中主要代谢产物和特殊代谢产物的重大变化。在这两个亚种中，感染导致糖（果糖，葡萄糖，蔗糖）的大量减少，而糖醇含量（甘露醇和阿拉伯糖醇）增加。关于氨基酸，小猪猪笼草的早期感染引起天冬氨酸，丝氨酸和天冬酰胺的减少，丙氨酸和α-丙氨酸的强烈增加。在Vv亚种中观察到了更强烈的主要代谢改变的趋势。 樟子松相比V.诉亚种。 葡萄。小叶猪笼草感染还引发了二苯乙烯和类黄酮化合物的重大变化。感染后两个亚种的木材中白藜芦醇和几种白藜芦醇低聚物的含量均增加。有趣的是，我们发现在Vv亚种中接种木材后，白藜芦醇低聚物（假定的E-宫本醇C，维提净C，希望酚，安培素C）含量更高。 樟子松。