A single-synapse resolution survey of PSD95-positive synapses in twenty human brain regions,European Journal of Neuroscience

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A single-synapse resolution survey of PSD95-positive synapses in twenty human brain regions
European Journal of Neuroscience ( IF 2.7 ) Pub Date : 2020-06-03 , DOI: 10.1111/ejn.14846
Olimpia E. Curran ₁ , Zhen Qiu ₁ , Colin Smith ₂ , Seth G.N. Grant _{1,

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Affiliation

Mapping the molecular composition of individual excitatory synapses across the mouse brain reveals high synapse diversity with each brain region showing a distinct composition of synapse types. As a first step towards systematic mapping of synapse diversity across the human brain, we have labelled and imaged synapses expressing the excitatory synapse protein PSD95 in twenty human brain regions, including 13 neocortical, two subcortical, one hippocampal, one cerebellar and three brainstem regions, in four phenotypically normal individuals. We quantified the number, size and intensity of individual synaptic puncta and compared their regional distributions. We found that each region showed a distinct signature of synaptic puncta parameters. Comparison of brain regions showed that cortical and hippocampal structures are similar, and distinct from those of cerebellum and brainstem. Comparison of synapse parameters from human and mouse brain revealed conservation of parameters, hierarchical organization of brain regions and network architecture. This work illustrates the feasibility of generating a systematic single-synapse resolution atlas of the human brain, a potentially significant resource in studies of brain health and disease.

中文翻译：

人类大脑 20 个区域 PSD95 阳性突触的单突触分辨率调查

绘制小鼠大脑中个体兴奋性突触的分子组成，揭示了突触的高度多样性，每个大脑区域显示出不同的突触类型组成。作为系统映射整个人脑突触多样性的第一步，我们标记并成像了 20 个人类大脑区域中表达兴奋性突触蛋白 PSD95 的突触，包括 13 个新皮质、2 个皮质下、1 个海马、1 个小脑和 3 个脑干区域，在四个表型正常的个体中。我们量化了单个突触点的数量、大小和强度，并比较了它们的区域分布。我们发现每个区域都显示出突触点参数的独特特征。大脑区域的比较表明皮质和海马结构相似，与小脑和脑干的不同。人类和小鼠大脑突触参数的比较揭示了参数的保守性、大脑区域的分层组织和网络结构。这项工作说明了生成人脑系统单突触分辨率图谱的可行性，这是研究大脑健康和疾病的潜在重要资源。

更新日期：2020-06-03

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