Tissue engineering using new strategies has become a growing and promising method for treating large tissue lesions in the body. On the other hand, microRNAs (miRNAs), which are small non‐coding regulatory RNAs, are a new class of genetic materials that can have effective pharmacological roles. The combination of these two themes has created promising prospects for the treatment of diseases. Herein, human induced pluripotent stem cells (iPSCs) were transduced with miRNA‐2861 and then the osteogenic differentiation potential of transduced iPSCs and non‐transduced iPSCs was investigated while cultured on the electrospun poly lactic‐co‐glycolic acid (PLGA) nanofibrous scaffold and culture plate. MiR‐2861‐transduced iPSCs showed a significantly higher viability, mineralization, alkaline phosphatase (ALP) activity, calcium content, and bone‐related gene expression in comparison with those iPSCs that non‐transduced. The results also indicated that this increase is improved when miR‐2861 transduced iPSCs are cultured on the PLGA nanofibrous scaffold synergistically. This synergy was also confirmed by the results obtained from of Western blot analysis. It can be concluded that, miR‐2861, by negative regulation of those proteins that decrease/inhibit osteogenic differentiation and PLGA nanofibrous scaffold by preparation of a suitable artificial extracellular matrix, have a great positive impact in improving iPSCs osteogenic differentiation potential and this blend can be proposed to use in bone tissue engineering application.

中文翻译：

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MicroRNA‐2861和纳米纤维支架协同促进人类诱导的多能干细胞成骨分化

使用新策略进行组织工程化已成为治疗体内大组织损伤的一种增长且有希望的方法。另一方面，microRNA（miRNA）是小的非编码调节性RNA，是一类新型的遗传材料，可以起有效的药理作用。这两个主题的结合为疾病的治疗创造了广阔的前景。在本文中，将人类诱导的多能干细胞（iPSC）用miRNA‐2861转导，然后在电纺聚乳酸-乙醇酸（PLGA）纳米纤维支架上培养转导的iPSC和非转导的iPSC的成骨分化潜能。培养板。MiR‐2861转导的iPSC的活力，矿化度，碱性磷酸酶（ALP）活性，钙含量，与未转导的iPSC相比，以及与骨骼相关的基因表达。结果还表明，当在PLGA纳米纤维支架上协同培养miR-2861转导的iPSC时，这种增加得到改善。通过蛋白质印迹分析获得的结果也证实了这种协同作用。可以得出结论，miR‐2861通过负向调节降低/抑制成骨细胞分化的蛋白质和通过制备合适的人工细胞外基质而对PLGA纳米纤维支架的调控，对改善iPSC的成骨细胞分化潜能具有巨大的积极影响，这种混合物可以被提议用于骨组织工程应用。结果还表明，当在PLGA纳米纤维支架上协同培养miR-2861转导的iPSC时，这种增加得到改善。通过蛋白质印迹分析获得的结果也证实了这种协同作用。可以得出结论，miR‐2861通过负向调节降低/抑制成骨细胞分化的蛋白质和通过制备合适的人工细胞外基质而对PLGA纳米纤维支架的调控，对改善iPSC的成骨细胞分化潜能具有巨大的积极影响，这种混合物可以被提议用于骨组织工程应用。结果还表明，当在PLGA纳米纤维支架上协同培养miR-2861转导的iPSC时，这种增加得到改善。通过蛋白质印迹分析获得的结果也证实了这种协同作用。可以得出的结论是，miR‐2861通过负向调节降低/抑制成骨细胞分化的蛋白质和通过制备合适的人工细胞外基质而对PLGA纳米纤维支架的调控，对改善iPSC的成骨细胞分化潜能具有巨大的积极影响，这种混合物可以被提议用于骨组织工程应用。